1.What is the primary instrument for viewing bacteria in a
microbiology lab?
A) Electron microscope
B) Compound
microscope
C) Dissecting microscope
D) Fluorescence microscope
B) Compound microscope
1.How is total magnification calculated in a compound microscope?
A) Objective + ocular
B) Objective x ocular
C)
Objective / ocular
D) Ocular - objective
B) Objective x ocular
1.What happens to the working distance as the magnifying power of the
lens increases?
A) It increases
B) It decreases
C)
It remains the same
D) It fluctuates
B) It decreases
1.What does RP (resolution) refer to in microscopy?
A)
Magnification power
B) Ability to distinguish two nearby points
as separate
C) Light intensity
D) Lens diameter
B) Ability to distinguish two nearby points as separate
1.What is the numerical aperture (NA) of a lens?
A) The
magnification power
B) The light-gathering capacity of the lens
C) The size of the lens
D) The wavelength of light used
B) The light-gathering capacity of the lens
1.How should a microscope be carried?
A) With one hand
B)
With both hands, one holding the arm and one under the base
C)
By the objective lens
D) By the stage
B) With both hands, one holding the arm and one under the base
1.What should be used to clean the oculars, objectives, and condenser
lenses?
A) Water
B) Alcohol
C) Dry lens paper
D) lens cleaner
D) Lens cleaner
1.What should be done at the end of each microscope session?
A)
Leave the slide on the stage
B) Remove the slide, wipe away the
oil, and place the scanning objective in a vertical position
C)
Turn off the light and leave the microscope on the bench
D)
Cover the microscope with a cloth
B) Remove the slide, wipe away the oil, and place the scanning objective in a vertical position
1.What is the purpose of staining in microbiology?
A) To kill
the bacteria
B) To increase contrast between bacteria and the
slide
C) To magnify the image
D) To change the color of
the bacteria
B) To increase contrast between bacteria and the slide
1.What is the first step in smear preparation from a plate or broth?
A) Apply stain to the slide
B) Begin with a clean, dry
slide
C) Heat fix the slide
D) Add immersion oil
B) Begin with a clean, dry slide
1.What is the purpose of heat fixing a slide?
A) To stain the
bacteria
B) To kill the bacteria and make them adhere to the
slide
C) To magnify the image
D) To clean the slide
B) To kill the bacteria and make them adhere to the slide
1.What stain is used in the simple stain procedure?
A) Congo
red
B) Methylene blue or Safranin
C) India ink
D)
Crystal violet
B) Methylene blue or Safranin
1.What is the purpose of the negative stain procedure?
A) To
stain the bacteria
B) To stain the background and leave the
bacteria unstained
C) To kill the bacteria
D) To magnify
the image
B) To stain the background and leave the bacteria unstained
1.What is used to spread the drop of bacteria and stain in the
negative stain procedure?
A) A pipette
B) The edge of a
second clean slide
C) A brush
D) A cotton swab
B) The edge of a second clean slide
1.What is the purpose of a wet mount?
A) To stain the bacteria
B) To observe living organisms in a liquid environment
C)
To kill the bacteria
D) To magnify the image
B) To observe living organisms in a liquid environment
1.What should be used to cover the drop of hay infusion liquid in a
wet mount?
A) A piece of paper
B) A second slide
C)
A coverslip
D) A glass rod
C) A coverslip