1.What charge do most bacterial stains have?
A) Negative
B) Positive
C) Neutral
D) Variable
B) Positive
1.What is the purpose of negative stains like Congo red and India
ink?
A) To stain the bacteria
B) To stain the background
C) To enhance bacterial growth
D) To kill the bacteria
B) To stain the background
1.What is the total magnification when using a 10x ocular and a 100x
objective?
A) 100x
B) 400x
C) 1000x
D) 10,000x
C) 1000x
1.At what magnification will you not see individual bacteria?
A) 100x
B) 200x
C) Less than 400x
D) More than 1000x
C) Less than 400x
1.What is the purpose of immersion oil when using the 100x objective?
A) To clean the slide
B) To improve resolution
C) To
magnify the image
D) To protect the lens
B) To improve resolution
1.What is the function of the condenser in a microscope?
A) To
magnify the specimen
B) To focus light on the specimen
C)
To adjust the stage
D) To change the objective lens
B) To focus light on the specimen
1.What does the term "parfocal" mean in microscopy?
A) The specimen will be out of focus when changing objectives
B) The specimen will almost be in focus when changing objectives
C) The light intensity will change when changing objectives
D) The magnification will change when changing objectives
B) The specimen will almost be in focus when changing objectives
1.How are Staphylococcus bacteria arranged?
A) Chains
B)
Pairs
C) Clusters of spherical bacteria
D) Single cells
C) Clusters of spherical bacteria
1.What is the function of immersion oil in microscopy?
A) To
clean the slide
B) To improve resolution by matching the
refractive index of glass
C) To magnify the image
D) To
protect the lens
B) To improve resolution by matching the refractive index of glass
1.What is the function of the condenser in a microscope?
A) To
magnify the specimen
B) To focus light on the specimen
C)
To adjust the stage
D) To change the objective lens
B) To focus light on the specimen
1.What is the function of the iris diaphragm in a microscope?
A) To magnify the specimen
B) To control the amount of
light passing through the condenser
C) To adjust the stage
D) To change the objective lens
B) To control the amount of light passing through the condenser
1.Why do you initially focus on the wax pencil mark instead of the
stain?
A) To see the bacteria more clearly
B) To ensure
the slide is in the correct position
C) To adjust the light
intensity
D) To clean the slide
B) To ensure the slide is in the correct position
1.What does the name Streptococcus refer to?
A) Rod-shaped
bacteria
B) Chains of spherical bacteria
C) Clusters of
spherical bacteria
D) Single spherical bacteria
B) Chains of spherical bacteria
1.Where do you discard used slides in the lab?
A) In the
regular trash
B) In a designated sharps container
C) In
the sink
D) In a recycling bin
B) In a designated sharps container