EXCERCISE 2 LAB MICROBIO Flashcards

1.What is meant by the term aseptic technique?
A) Techniques to speed up bacterial growth
B) Techniques to avoid contamination
C) Techniques to kill bacteria
D) Techniques to identify bacteria

1.Where should you label your agar plates and how should they be stored?
A) On the lid; stored right side up
B) On the bottom; stored upside down
C) On the side; stored on their side
D) On the lid; stored upside down

1.Why should you wait for your loop to completely cool before attempting a bacterial transfer?
A) To avoid burning your hand
B) To avoid killing the bacteria
C) To make the transfer faster
D) To prevent contamination

1.How do you prevent air contamination of agar plates if they are not flamed?
A) Cover them with a cloth
B) Keep the lid partially closed
C) Store them in a refrigerator
D) Use a sterile container

1.When you remove the lid of a test tube or an agar plate, it is OK to set it on a clean workbench while you remove or add bacteria.
A) True
B) False

1.The opening of the test tubes and the lid should be passed through the flame before the lid is replaced on the tube.
A) True
B) False

1.It is OK for one group member to hold a test tube while another member extracts bacteria from it for a transfer.
A) True
B) False

1.What should be done to the workspace before starting bacterial transfers?
A) Use bleach wipes or alcohol wipes
B) Clean it with bench disinfectant or bleach and remove clutter
C) Cover it with paper towels soaked with bench disinfectant
D) Spray it with ethanol

1.Why should bacterial transfers be done close to the flame of a Bunsen burner?
A) To see better
B) To reduce contamination from airborne microbes
C) To keep warm
D) To sterilize the air

1.How should test tubes be handled to prevent spills?
A) Hold the tube with metal tongs
B) Hold the tube with a tube holder
C) Hold the tube and not the cap
D) All of the above

1.What is the correct way to mix broth cultures before transfer?
A) Shake them vigorously
B) Roll them back and forth between your palms
C) Stir with an inoculating loop
D) Swirl the tube in hand

1.What should be done to the inoculating loop before picking up cells?
A) Dip it in alcohol
B) Allow it to cool completely after flaming
C) Wipe it with a tissue
D) Heat it in the microwave

1.How should the cap of a test tube be handled during a bacterial transfer?
A) Lay it down on the table
B) Grasp it between the ring and pinky fingers and the palm
C) Hold it with tweezers
D) Place it in a sterile container

1.What should be done if a spill occurs during a bacterial transfer?
A) Cover it with a paper towel soaked in ethanol
B) Wipe it with a dry cloth
C) Clean it up immediately with 10% bleach or bench disinfectant
D) Cover it with a paper towel soaked in bench disinfectant

1.How should bacterial cultures be labeled?
A) On the lid of the plate
B) On the bottom of the plate
C) On a separate piece of paper
D) On the side of the tube

1.What is the purpose of the ubiquity exercise?
A) To learn how to use a microscope
B) To illustrate the variety of bacteria in the environment
C) To study the effects of antibiotics
D) To measure bacterial growth rates

1.What is used to isolate bacteria from a source of your choosing?
A) A pipette
B) A sterile needle
C) A dry sterile swab
D) A glass slide

1.What can be used for the initial isolation of your sample besides a dry sterile swab?
A) A moist swab
B) A broth culture
C) A Petri dish
D) A microscope slide

1.What should you do after dipping the sterile swab into sterile saline or water?
A) Shake it vigorously
B) Push the swab against the side of the test tube to squeeze excess water
C) Leave it in the tube
D) Dry it with a paper towel

1.What type of agar is used for streaking plate technique?
A) Nutrient agar
B) Blood agar
C) Trypticase soy agar
D) MacConkey agar
E) Nutrient or trypticase agar

1.At what temperature should the plates be incubated?
A) 25°C
B) 30°C
C) 37°C
D) 42°C

1.For how long should the plates be incubated?
A) 12 to 24 hours
B) 24 to 36 hours
C) 24 to 48 hours
D) 48 to 72 hours

1.What should you do after the incubation period?
A) Discard the plates
B) Add more bacteria
C) Observe the plate for growth
D) Store the plates in the refrigerator

1.What is the primary purpose of the Gram stain technique?
A) To measure bacterial growth
B) To differentiate between Gram-positive and Gram-negative bacteria
C) To identify bacterial spores
D) To determine bacterial motility

1.Which of the following is the primary stain used in the Gram stain technique?
A) Safranin
B) Crystal violet
C) Methylene blue
D) Carbol fuchsin

1.What is the role of iodine in the Gram stain process?
A) To decolorize the cells
B) To act as a mordant and fix the crystal violet stain
C) To counterstain the cells
D) To wash off excess stain

1.What color do Gram-positive bacteria appear after the Gram stain procedure?
A) Red
B) Purple
C) Green
D) Colorless

1.What cell wall component does the decolorizing agent in the Gram stain technique effect?
A) Teichoic acid
B) Peptidoglycan
C) Lipopolysaccharide
D) Myolic acid

1.Which of the following is used as the counterstain in the Gram stain technique?
A) Crystal violet
B) Safranin
C) Iodine
D) Methylene blue

1.What is the typical shape of cocci bacteria?
A) Rod-shaped
B) Spherical
C) Spiral
D) Filamentous

1.How are bacteria arranged in a "streptococci" formation?
A) In clusters
B) In chains
C) In pairs
D) In single cells

1.What is the approximate size range of most bacteria?
A) 0.1 to 1 micrometer
B) 1 to 10 micrometers
C) 10 to 100 micrometers
D) 100 to 1000 micrometers