These structures are used by bacteriophages to attach to host cell receptors.
tail fibers
True or False: Prophages can be activated into viral replication and enter the lytic cycle.
True
Viruses with __________ sense RNA contain the correct message for translation, while viruses with _________ sense RNA must first be converted into a correct message.
positive, negative
The capsomers are made of
protein.
Which of the following will not support viral cultivation?
blood agar
Clostridium botulinum is made virulent by incorporated prophage genes encoding for the botulinum toxin. What term describes this process?
Lysogenic conversion
In general, most DNA viruses multiply in the host cell's _____, while most RNA viruses multiply in the host cell's _____.
nucleus, cytoplasm
Freshly isolated animal tissue that is placed in a growth medium and allowed to produce a cell monolayer is referred to as a cell culture.
primary
All of the following is correct about treating viral diseases except
viruses are killed by the same antibiotics that kill bacteria.
Viruses have all the following except
metabolism.
True or False: The International Committee on Taxonomy of Viruses has determined that there are only three orders of viruses.
False
True or False: Viruses are ultramicroscopic because they range in size from 2 mm to 450 mm.
False
The correct sequence of events in viral multiplication is
adsorption, penetration, uncoating, synthesis, assembly, release.
True or False: Bacteriophages do not undergo adsorption to specific host cell receptors prior to penetration.
False
Viral growth in bird embryos can cause discrete, opaque spots in the embryonic membranes called
pocks.
Viruses belong to which of the following Kingdoms?
None of the choices are correct.
Oncogenic viruses include all the following except
Measles virus.
Which of the following viruses did D. Ivanovski and M. Beijerinck work with?
Tobacco Mosaic Virus
How do enveloped animal viruses exit their host?
Budding or exocytosis
True or False: Spikes are glycoproteins of the virus capsid.
False
Viruses that infect bacteria are specifically called _____.
bacteriophages
Virus capsids are made from subunits called
capsomeres.
Uncoating of viral nucleic acid
All of the choices are correct.
The virus-induced, specific damage to the host cell that can be seen in a light microscope is called
cytopathic effects.
Satellite viruses are
dependent on other viruses for replication.
Visible, clear, well-defined patches in a monolayer of virus-infected cells in a culture are called
plaques.
Creutzfeld-Jacob disease is
a spongiform encephalopathy of humans.
True or False: The adeno-associated virus (AAV) and the delta agent are prions.
False
Classification of viruses into families involves determining all the following characteristics except
biochemical reactions.
Which of the following represents a virus family name?
Herpesviridae
Host cells of viruses include
All of the choices are correct.
A(n) _____ is the protein shell around the nucleic acid core of a virus.
capsid
True or False: Viral spikes are inserted into the host cell membrane before budding or exocytosis occurs.
True
A naked virus does not have a(n) ________ .
envelope
True or False: No cases of variant Creutzfeldt-Jakob syndrome linked to eating infected cows have occurred in the United States.
True
True or False: When a virus enters a host cell, the viral genes redirect the genetic and metabolic activities of the host cell.
True
The core of every virus particle always contains
either DNA or RNA.
Which of the following is correct
about viruses?
cannot be seen with a light microscope
When a virus enters a lysogenic phase, it means
the virus is integrated into the DNA of the host cell and is latent.
All of the following pertain to virus envelopes except they
are located between the capsid and nucleic acid.
Two noncellular agents, smaller than viruses, are the infectious proteins called _______ , and the infectious RNA strands called ________ .
prions, viroids
Which of the following is a type of cytopathic effect?
All of the choices are correct.
T-even phages
infect Escherichia coli cells.
Viruses that cause infection resulting in alternating periods of activity with symptoms and inactivity without symptoms are called
latent
True or False: Viral infections are easier to treat with drugs than bacterial infections.
False
Diagnosis of viral infections sometimes involves analyzing the patient's blood for specific _____ that the immune system produced against the virus.
Antibodies
True or False: A closed culture system is used to determine a population growth curve.
True
The E. coli that normally live in the human large intestines and produce vitamin K that the body uses would be best termed a _____ relationship.
Mutualistic
Organisms that feed on dead organisms for nutrients are called
Saprobes
The time interval from parent cell to two new daughter cells is called the
Generation time
What type of media is used to demonstrate oxygen requirements of microbes?
Thioglycollate
The methanogens, producers of methane gas, require environments that
Are anaerobic with hydrogen gas and CO2
The use of energy by a cell to enclose a substance in its membrane by forming a vacuole and engulfing it is called
Endocytosis
The toxic superoxide ion is converted to harmless oxygen by two enzymes,
Superoxide dismutase and catalase
An organism that cannot grow without oxygen is a(n)
Obligate aerobe
Aerobic respiration is an example of
Chemoheterotrophy
The movement of substances from higher to lower concentration across a semi permeable membrane that must have a specific protein carrier but no energy expenditure is called
Facilitated diffusion
True or False: A saprobe with a cell wall will utilize extracellular digestion.
True
The phase of the bacterial growth curve in which the rate of multiplication equals the rate of cell death is the
Stationary phase
True or False: Anaerobes can be cultured in a CO2 environment.
True
True or False: Obligate saprobes can adapt to a living host.
False
All of the following could find a location in or on body tissues suitable for growth except
Psychrophiles
A halophile would grow best in
Salt lakes
An organic nutrient that cannot be synthesized by the organism and must be provided is called a/an
Growth factor
The phase of the bacterial growth curve that shows the maximum rate of cell division is the
Log phase
True or False: Facilitated diffusion and active transport require a carrier protein to mediate the movement across the plasma membrane.
True
In _____ conditions, the cell wall will help prevent the cell from bursting.
Hypotonic
True or False: Whether an organism is an autotroph or heterotroph depends on its source of nitrogen.
False
True or False: After binary fission, daughter cells will differ genetically.
False
Microorganisms that live in severe habitats, such as very hot, acidic or salty environments, are called
Extremophiles
When it is important to count the number of cells, determine cell size and differentiate between dead and live cells a ___ is used.
flow cytometer
Cultures of a bacterial species were incubated on the shelf of a refrigerator, out on a lab bench top, on the shelf of a 37° C incubator and on the shelf of a 50° C incubator. After incubation, there was no growth at 37° C and 50° C, slight growth out on the bench top and abundant growth at refrigeration. What term could be used for this species?
Psychrophile
The production of antibiotics is a form of antagonism called
Antibiosis
True or False: The time that it takes for a freshly inoculated agar culture to develop visible colonies is principally governed by that species' generation time.
True
Bacteria living in a freshwater stream that are moved to salty seawater would
Shrivel
Contractile vacuoles are
Used to expel excess water from cells
Organisms called _____ live on or in the body of a host and cause some degree of harm.
Pathogens
When whole cells or large molecules in solution are engulfed by a cell, this endocytosis is specifically termed
Phagocytosis
An organism that can use gaseous oxygen in metabolism and has the enzymes to process toxic oxygen products is a(n)
Aerobe
True or False: Most microorganisms on earth can only live and survive in habitats that are similar to human body conditions.
False
Calcium is required for bacteria because
It stabilizes the cell wall
An important mineral ion that is a component of chloroplasts and stabilizer of membranes and ribosomes is
Magnesium
Cultures of a bacterial species were incubated out on the incubator shelf, in an anaerobic jar and in a candle jar. After incubation there was moderate growth of cultures in the candle and anaerobic jars, but heavy growth of the culture on the incubator shelf. This species is a/an
Facultative anaerobe
An organism that can exist in both oxygen and oxygen-less environments is a(n)
Facultative anaerobe
True or False: Inorganic nitrogen must be converted to ammonia to be used by a cell.
True
Growth factors
Cannot be synthesized by the organism
The movement of substances from lower to higher concentration across a semi permeable membrane that must have a specific protein carrier and cell expenditure of energy is called
Active transport
Diffusion of water through a semi permeable membrane is called
Osmosis
The phase of the bacterial growth curve in which newly inoculated cells are adjusting to their new environment, metabolizing but not growing is the
Lag phase
A microorganism that does not have catalase or superoxide dismutase would find it difficult to live in an environment with
Oxygen
Microorganisms require large quantities of this nutrient for use in cell structure and metabolism
Macronutrient
The term facultative refers to
The ability to exist in a wide range of conditions
Why are Archea as a group are not pathogens?
Because mammalian hosts do not meet their environmental requirements
The term phototroph refers to an organism that
Gets energy from sunlight
True or False: Bacteria have an average generation time of 24 hours.
False
True or False Whether an organism is a phototroph or a chemotroph depends on its source of energy.
True
What is a virus?
an infectious particle consisting of gene packaged in a protein coat
What did Ivanoski and Beijerink do?
discovered and isolated tobacco mosaic virus
Viruses have DNA ____(and/or)___ RNA
DNA or RNA NEVER BOTH
What is the most abundant microbe on earth?
viruses
Are viruses cellular or noncellular?
Noncellular meaning they are non living
Can viruses metabolize?
No viruses cannot metabolize
Are viruses capable of replicating without a cellular host?
No, viruses are obligate meaning they require a host to replicate
Can viruses form their own structures and receptors?
No, they have no machinery for protein synthesis
What is a capsid?
a viral protein coat
What is the capsid together with the nucleic acid called?
nucleocapsid
What are the identical protein subunits of a capsid called?
capsomeres
Give some examples of enveloped viruses
HIV, Coronavirus, Yellow fever, Rubella, Hepatitis C
What are the two structural capsid types
Helical - continuous helix of capsomers
Icosahedral - 3D, 20-sided with 12 corners
Why can enveloped viruses not survive long outside of a host?
The lipid bilayer is prone to drying out
What are the capsid and envelope responsible for?
transfer of the viral genetic material from one cell to another, resistance to chemical or physical inactivation
What are spikes essential for?
for attachment of viruses to the host cell
Give an example of a plant virus that is enveloped
Tomato spotted wilt virus
_____________ viruses possess great adaptability and can change in a short time in order to invade the immune system
Enveloped
What kind of virus uses bacteria as its host
bacteriophages
What are the general phases in animal virus multiplication cycle
Adsorption
Penetration
Uncoating
Synthesis
Assembly
Release
Give examples of DNA viruses
Papillomavirus, Herpesviruses, Epstein-Barr, Hepatitis B
Give examples of RNA viruses
HIV, COVID, FLU, Measles, Rabies
ssRNA genomes ready for immediate translation are
positive sense RNA
ssRNA genomes that must be converted into proper form are
negative sense RNA
what is needed to convert negative sense RNA to positive sense RNA
reverse transcriptase
Family names of viruses end in
viridae EX: Herpesviridae
Genus name of viruses ends in
-virus EX: Simplexvirus
What is endocytosis
entire virus is engulfed and enclosed in a vacuole or vesicle
What is fusion
envelope merges directly with membrane resulting in nucleocapsids entry into cytoplasm
Where are DNA viruses assembled
the nucleus
Where are RNA viruses assembled
the cytoplasm
What are the two ways viruses can leave a host
Budding - sheds viruses gradually, cell is not immediately destroyed
Lysis - released when cell dies and ruptures
Define Cytopathic effect (CPE)
virus induced specific damage to cell that alters its microscopic appearance
Define persistent infections and give examples
cell harbors the virus and is not immediately lysed, Measles, Herpes simplex, Herpes zoster
Define oncoviruses and give examples
Mammalian viruses capable of initiating tumors examples are Epstein Barr Virus, Hepatitis B, Hepatitis C, HIV
What are the stages of bacteriophage replication in order
Adsorption, Penetration, Replication, Assembly, Maturation, Lysis and Release
What are the two life cycles of a bacteriophage
lytic and lysogenic
What kind of phages undergo adsorption and penetration but don't replicate
temperate phages
The viral genome inserts into bacterial genome and becomes an inactive __________.
prophage
When a prophage is retained and copied during normal cell division resulting in the transfer of temperate phage genome to all host cell progeny is called?
lysogeny
______________ can occur resulting in activation of lysogenic prophage followed by viral replication and cell lysis
Induction
Lysogeny results in the spread of the virus without ___________ the host cell
killing
What is lysogenic conversion
phage genes in the bacterial chromosome can cause the production of toxins or enzymes that cause pathology
What methods can you use to grow viruses
cell (tissue) cultures
Birid embryos
Live animal inoculation
True or False: Viruses are obligate intracellular parasites
True
What are prions
proteinaceous infectious particles - infectious misfolded proteins, contain no nucleic acid
What are some prion diseases common in animals
Scrapie - in sheep and goats
Mad Cow Disease (Bovine spongiform encephalopathies)
Wasting disease - in elk
Give examples of Human prion diseases
Kuru
Creutzfeldt-Jakob disease (CJD)
Fatal familial insomnia (FFI)
Describe Satellite viruses
dependent on other viruses for replication
Describe viroids
tiny circular, single-stranded RNAs that are infectious pathogens. Have no protein coating, common in plants may also affect humans
What are essential nutrients
must be provided to an organism can not acquire denovo
96% of cell is composed of 6 elements name those elements
carbon
hydrogen
oxygen
phosphorous
sulfer
nitrogen
Heterotroph
depend on other living things
Autotroph
uses CO2 does not depend on other living things
Chemotroph
gain energy from chemical compounds
Phototroph
gain energy through photosynthesis
Saprobes
free-living microorganisms that feed on organic detritus from dead organisms
Parasites
derive nutrients from host
Passive Transport
does not require energy, higher concentration to lower EX: diffusion, osmosis, and facilitated diffusion
Active Transport
requires energy and carrier proteins EX: group translocation, bulk transport
Isotonic
equal inside and out in both Bacteria and Humans
Hypotonic
into cell causes; in Humans it causes it to burst or swell in Bacteria the wall prevents it from bursting
Hypertonic
out of cell; in Humans causes it to shrink and become distorted in Bacteria shrinks cell membrane away from cell wall
Endocytosis
bringing substances into the cell through a vesicle or phagosome
Phagocytosis
ingests substances or cells
Pinocytosis
ingests liquids
What is a niche when referring to bacteria
the totality of adaptions organisms make to their habitat including factors such as pH, temperature, oxygen requirements, osmotic pressure, and barometric pressure
psychrophiles
< 15 ℃
mesophile
20 - 40 ℃
thermophiles
> 45 ℃
Enzymes that function at high temperatures or other harsh conditions are called:
extremozymes
What does Thermoduric mean
survive for short time above maximum temperature for growth EX: are Bacillus, Microbacterium, Streptococcus
enzymes that kill toxic oxygen products
superoxide, dismutase, catalase
utilizes oxygen and can detoxify it
aerobe
cannot grow without oxygen aerobe EX: Micrococcus luteus
Obligate aerobe
utilizes O2 if it is available, but can also grow in the absence of O2 EX: Staphylococci
Facultative anaerobe
requires only a small amount of oxygen Helicobacter pylori
Microaerophilic
does not utilize oxygen
Anaerobe
lacks the enzymes to detoxify O2 so can not survive in an O2 environment EX: Clostridium
Obligate anaerobe
do not utilize O2 but can survive and grow in its presence EX: Lactobacilli
Aerotolerant anaerobes
Neisseria gonorrhoeae
example of facultative anaerobe Gram-negative coffee-bean "diplococci"
Capnophile
grows best at higher CO2 tensions than normally present in the atmosphere EX: Haemophilus influenza
require a high concentration of salt
Halophiles
do not require high concentration of solute but can tolerate it when it occurs
Osmotolerant
can survive under extreme pressure and will rupture if exposed to normal atmospheric pressure
Basophiles
Biofilms
cooperative aggregate of microorganisms associated a surface and enclosed in a polymeric matrix
quorum sensing
communicate and cooperate in the formation and functions of biofilms
Beneficial biofilms
layers of normal microbiota lining the intestinal and respiratory mucosa help ward off infections by pathogens
Harmful biofilms
form on teeth can contribute to dental and periodontal diseases, forms on wounds causing infections that can spread
Microbial growth occurs at two levels growth at a cellular level with increase in ________ and increase in ____________.
size, population
Division of bacterial cells occurs mainly through ______________
binary fission
Time required for a complete fission cycle is called the ________________, or doubling time
generation
Each new fission cycle increases the population by a factor of ______.
2
Rate of population growth forumla
Nt = (Ni)2n
What are the 4 stages of the population growth curve
Lag phase, Log (exponential) phase, Stationary phase, Death phase
What stage is best for Gram staining
log (exponential) phase
What stage is best for motility testing
log (exponential) phase
What stage is best for endospore staining
death phase
Define metabolism
all chemical and physical workings of a cell
Define Anabolism
forms larger macromolecules from small molecules, biosynthesis, requires energy
Define Catabolism
breaks larger molecules to form smaller molecules, biodegeneration, releases energy
What is an enzyme
a biological catalyst
Define exoenzymes and give examples
transported extracellularly, where they break down large food molecules or harmful chemicals, play a role in toxins EX: cellulase, amylase, penicillinase
Define endoenzymes
retained intracellularly and function there, play a role in metabolic pathways (most enzymes fall into this category)
Define labile
chemically unstable enzymes
Define denaturation
weak bonds that maintain the shape of the apoenzyme are broken
Substances that resembles the normal substrate competes with the substrate for the active site
Competitive inhibition
enzymes are regulated by the binding of molecules other than the substrate away from the active site
Noncompetitive inhibition
What are the 3 linked pathways of catabolism
Glycolysis, Kreb's cycle, Electron transport chain
Bioenergetics
study of the mechanisms of cellular energy release
Aerobic respiration
glycolysis, the Kreb's cycle, respiratory chain (MOST ENERGY)
Anaerobic respiration
glycolysis, the Kreb's cycle, respiratory chain; O2 is not the final electron acceptor
Fermentation
glycolysis, organic compounds are the final electron acceptors (LEAST ENERGY)
limiting factors intensify, cells die exponentially
Death phase
grow at extreme acid pH
Acidophiles
grow at extreme alkaline pH
Alkalinophiles