Which question correctly compares prokaryotes and eukaryotes with
respect to
genome organization (select one):
A. Prokaryotes
have less DNA compared to eukaryotes and more of it is
coding
sequence.
B. Both prokaryotes and eukaryotes have
non-coding sequences in their
genomes.
C. The genomes for
prokaryotes are usually circular, the genomes for
eukaryotes are
usually linear.
D. Intronic regions are mostly associated with
eukaryotes.
E. All of the above.
all
Which of the following best describes chromatin (select one)?
A.
Circular DNA wrapped tightly around histones.
B. Condensed linear
DNA attached to microtubules during mitosis.
C. Linear DNA wound
around histones and non-histone proteins.
D. mRNA transcripts
passing through ribosomes in the cytoplasm being
translated into
protein.
E. DNA that is packaged tightly in the nucleus
linear dna around histone and non histone
You are testing a new drug to determine its effect on chromatin
structure. Your
results indicate you have more heterochromatin in
the drug treated sample
compared to your control. Considering
this result, which of the following is a
possible mechanism of
your drug (select one)?
A. It might inhibit a kinase which
phosphorylates serine, threonine, or tyrosine
on
histones.
B. It might inhibit a phosphatase which
dephosphorylates phosphorylated serine,
threonine, or
tyrosine.
C. It might inhibit a histone deacetylase (HDA)
D.
It might methylate DNA
E. It might demethylate DNA
might inhibit kinase which phosphoroylates
Histone methylation may lead to the silencing of gene expression.
Which of the
following is a plausible reason for why? (select all
that apply)
A. methylation competes with acetylation
B.
methylation competes with phosphorylation
C. methylation reduces
the charge on lysine
D. methylation recruits proteins that
enhance heterochromatin structure
E. All of the above
competes with acetylation
recruits proteins to enhance heterochromatin
Tyrosine, threonine, and serine can be phosphorylated on their side
chains.
Which of the following functional groups are replaced by
this modification (select
one)?
A. Sulfhydryl
B.
Hydroxyl
C. Phosphoryl
D. Amine
E. Amide
hydroxyl
DNA Replication is ____ (select one)?
A. Conservative and
unidirectional
B. Semi-conservative and bi-directional
C.
Unidirectional and semiconservative
D. Bidirectional and
conservative
E. Catalyzed by RNA Polymerase
semi conservative; bidirectional
What term is given to DNA binding proteins that promote gene
expression by
recruiting/helping RNA polymerase to bind promoters
transcription factors
Primase is essential for in vivo DNA replication because
synthesizes RNA component of primers
privides something for DNA polymerase to add bases to
what is true about helicase
triggired when origin fires
creates seperate DNA strands in replication
How does “the cell” solve the problem of replicating the 3’ to 5’ DNA strand
lagging strand is replicated in short incriments and later joined
Which of the following is true about telomerase (select all)?
A.
It is a ribonucleoprotein (a protein that has an RNA and DNA
component)
B. It adds telomeric repeat sequences to the 3’ end of
DNA strand that served
as the template in the last round of
replication.
C. It is most functional in somatic (body) cells,
and less functional in stem cells.
D. It contributes to cellular
life span and helps regulate cellular senescence
(when cells stop
growing).
E. It has a circular shape, which prevents the ends of
chromosomes from being
ligated together.
ribonucleoprotein, adds telomeric repeat on 3', contributes to cellular life span
where do histone mods occur
N terminal on histone tails
Which term describes the region of a plasmid where many restriction sites are found
multiple clone
How can eukaryotic genes be cloned for expression in a prokaryote
enzyme convert mRNA to cDNA