Biochem- Glycolysis Steps Flashcards

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1

Step 1

Phosphorylation of glucose

2

Step 1: Phosphorylation of glucose

Enzyme:

Hexokinase

3

Step 1: Phosphorylation of glucose

Delta G:

-16.7 kJ/mol

4

Step 2

Isomerization of glucose 6-phosphate

5

Step 2: Isomerization of glucose 6-phosphate

Enzyme:

Phosphohexose isomerase

6

Step 2: Isomerization of glucose 6-phosphate

Delta G:

1.7 kJ/mol

7

Step 3

Phosphorylation of fructose 6-phosphate

8

Step 3: Phosphorylation of fructose 6-phosphate

Enzyme:

Phosphofructokinase-1 (PFK-1)

9

Step 3: Phosphorylation of fructose 6-phosphate

Delta G:

-14.2 kJ/mol

10

Step 4

Cleavage of fructose-1,6-bisphosphate

11

Step 4: Cleavage of fructose-1,6-bisphosphate

Enzyme:

Aldolase

12

Step 4: Cleavage of fructose-1,6-bisphosphate

Delta G:

23.8 kJ/mol

13

Step 5

Isomerization of triose phosphate

14

Step 5: Isomerization of triose phosphate

Enzyme:

Triose phosphate isomerase

15

Step 5: Isomerization of triose phosphate

Delta G:

7.5 kJ/mol

16

Step 6

Oxidation of glyceraldehyde-3-phosphate

17

Step 6: Oxidation of glyceraldehyde-3-phosphate

Enzyme:

Glyceraldehyde 3-phosphate dehydrogenase

18

Step 6: Oxidation of glyceraldehyde-3-phosphate

Delta G:

6.3 kJ/mol

19

Step 7

Substrate level phosphorylation of ADP

20

Step 7: Substrate level phosphorylation of ADP

Enzyme:

Phosphoglycerate kinase

21

Step 7: Substrate level phosphorylation of ADP

Delta G:

-18.5 kJ/mol

22

Step 8

Conversion of 3-P-glycerate to 2-P-glycerate

23

Step 8: Conversion of 3-P-glycerate to 2-P-glycerate

Enzyme:

Phosphoglycerate mutase

24

Step 8: Conversion of 3-P-glycerate to 2-P-glycerate

Delta G:

4.4 kJ/mol

25

Step 9

Dehydration of 2-P-glycerate to PEP

26

Step 9: Dehydration of 2-P-glycerate to PEP

Enzyme:

Enolase

27

Step 9: Dehydration of 2-P-glycerate to PEP

Delta G:

7.5 kJ/mol

28

Step 10`

Phosphate group transfer from PEP to ADP

29

Step 10: Phosphate group transfer from PEP to ADP

Enzyme:

Pyruvate kinase

30

Step 10: Phosphate group transfer from PEP to ADP

Delta G:

-31.4 kJ/mol