What differentiates a eukaryotic mRNA strand from its precursor
RNA?
A. Introns are spliced out
B. Exons are spliced
out
C. Methylation and other epigenetic factors are
manipulated
D. There is no difference
A. Introns are spliced out
T/F: 5’ to 3’ of mRNA corresponds to N-to-C terminus in
polypeptides
A. True
B. False
A. True
List other names for the non-template strand of DNA.
Coding strand, RNA-like strand, sense strand
Which two are the correct pairings of promotes sequences and their
locations?
A. TATAAT (Pribnow box), -35 bp
B. TATAAT
(Pribnow box), -10 bp
C. TTGACA, -35 bp
D. TTGACA, -10 bp
B. TATAAT (Pribnow box), -10 bp
C. TTGACA, -35 bp
If the template strand of DNA is 5’ATTGGTAGCT3’, what is the sense strand? What is the resulting mRNA strand?
Sense strand: 5’AGCTACCAAT3’
mRNA: 5’AGCAUGGAAU3’
Which of the following is NOT true about sigma factors?
A.
Eukaryotic RNA Polymerase recognizes it
B. It recognizes certain
specific sequences on the promoter
C. It signals the start of
transcription
D. It is required by RNA polymerase to bind to the
DNA sequence
A. Eukaryotic RNA Polymerase recognizes it
T/F: Promotors can vary in strength
A. True
B. False
A. True
Which of the following is NOT true regarding initiation of
prokaryotic transcription?
A. Sigma factor identifies the
promotor region, allowing RNA polymerase
holoenzyme to bind to
promoter
B. RNA polymerase holoenzyme unwinds the DNA to make a
transcription
bubble
C. Sigma factor remains bound to the
RNA polymerase complex throughout the transcription process
D.
All of the above are true
C. Sigma factor remains bound to the RNA polymerase complex throughout the transcription process
T/F: Only one RNA polymerase can bind to a particular DNA strand at a
time.
A. True
B. False
B. False
Describe Rho-dependent termination of prokaryotic transcription.
- Rho factor binds to the rut site on RNA transcript and moves
towards 3’ end of the transcript
- RNA polymerase reached the
termination sequence and pauses transcription
- Rho factor
catches up and uses helices like activity to cleave the DNA-RNA hybrid
ending transcription
T/F: Both Rho independent and dependent termination involve reaching
a
transcription sequence roughly 40 nucleotides long.
A.
True
B. False
A. True
Which of the following is true regarding Rho-independent termination
of prokaryotic
transcription
A. The DNA sequence being
transcribed contains inverted repeats
B. The RNA molecule forms a
hairpin structure
C. There is usually a polyA sequence following
the inverted repeats on teh DNA
strand
D. All of the above
are true
D. All of the above are true
T/F: Eukaryotic mRNA is usually polycistronic in nature
A.
True
B. False
B. False
Which of the following is FALSE regarding eukaryotic
transcription?
A. In addition to core promoter sequences,
eukaryotic DNA also contains
regulatory promoter sequences and
enhancers and silencers which regulate
transcription
rate.
B. The core promoter is located at the -10 region
C.
There are 5 types of RNA polymerases in eukaryotes
D. RNA
Polymerase II is mainly responsible for mRNA synthesis in eukaryotes
B. The core promoter is located at the -10 region
Which of the following is true regarding eukaryotic
transcription?
A. Multiple transcription factors bind to RNA
Polymerase II
B. TATA box binding protein (TBP), which is
contained within TFIID is crucial for
the identification and
binding of RNA Polymerase to the core promoter.
C. After 30 bp,
RNA polymerase continues transcription without the rest of
its
machinery
D. All of the above are true
D. All of the above are true
Describe how RNA Polymerases I-III terminate transcription in Eukaryotes:
I: requires a termination factor similar to rho. Binds to DNA here NOT RNA!!
ll:Termination of transcription has no specific
sequences.
cleavage takes place at 3’ end of pre-mRNA (while
transcription is still going on)
Rat1 exonuclease starts to
degrade mRNA sequence still on DNA
Rat1 reaches RNA polymerase
and transcription is stopped
III: ends transcription after terminator sequence w/multiple Us
Which FOUR statements below are correct?
A. Both prokaryotic and
eukaryotic mRNAs have a 5’ cap
B. Only prokaryotic mRNAs are
polyadenylated at the 3’ end
C. In prokaryotes, transcription is
coupled to translation
D. Editing of the nucleotides in the
pre-mRNA strand is possible and would
change resulting amino acid sequence
E. In Eukaryotes, RNA splicing occurs after the mRNA is transported
into the
cytoplasm
F. RNA splicing requires the formation of
a spliceosome
G. Both prokaryotic and eukaryotic mRNAs are
synthesized by RNA
polymerase
C. In prokaryotes, transcription is coupled to translation
D.
Editing of the nucleotides in the pre-mRNA strand is possible and
would
change resulting amino acid sequence
F. RNA splicing requires the formation of a spliceosome
G. Both
prokaryotic and eukaryotic mRNAs are synthesized by RNA
polymerase
T/F: In eukaryotes, one pre-mRNA strand can only result in one
protein.
A. True
B. False
B. False - alternative splicing!
T/F: The promotor region is located downstream of the transcription
start site?
A. True
B. False
B. False - upstream!
T/F: mRNA is transcribed from the sense strand of DNA, making the
two
complementary.
A. True
B. False
B. False - antisense!
Carefully read the following double stranded DNA. Locate the
consensus sequence in
these strands and then answer the following
questions.
5’ GCATTATAGCCAAATCTTCAGA 3'
3’
CGTAATATCGGTTTAGAAGTCT 5’
21. What is this consensus sequence called?
Pribnow Box
Carefully read the following double stranded DNA. Locate the
consensus sequence in
these strands and then answer the following
questions.
5’ GCATTATAGCCAAATCTTCAGA 3'
3’
CGTAATATCGGTTTAGAAGTCT 5’
22. Is this DNA prokaryotic or eukaryotic?
Prokaryotic
Carefully read the following double stranded DNA. Locate the
consensus sequence in
these strands and then answer the following
questions.
5’ GCATTATAGCCAAATCTTCAGA 3'
3’
CGTAATATCGGTTTAGAAGTCT 5’
23. Where is it located relative to the start of transcription?
10 Nucleotides upstream
24. What is this sequence’s equivalent in either prokaryotes or eukaryotes?
TATA box
25. Which of the following causes DNA and RNA to separate during Rho
dependent
termination?
A. Rho’s exonuclease activity
B.
Rho’s helicase activity
C. The A-U bond breaks
D. RNA
Polymerase cleaves the mRNA off
B. Rho’s helicase activity