Microbiology: Lab Exercises (1-7) Flashcards

Which container would you put a used coverslip in?

Which container would you put used gloves in?

Which container would you place a razor in?

Which piece of glassware has an equal circumference were the opening has a much smaller circumference?

Which piece of glassware has a progressively smaller circumference from the bottom to the top?

Which machine is used to heat a mixture and stir a mixture using electromagnetic forces?

Which piece of equipment is used to sterilize equipment and media?

Which piece of equipment is used to avoid contamination when working with microbes?

Which piece of equipment is used to measure the amount of material based on absorbance?

Which machine is used to perform polymerase chain reactions?

Which machine is used to keep liquids at a constant temperature?

Which machine is used to grow and maintain microorganisms at optimal physical conditions?

Which tool is used to move microorganisms between cultures and can be repeatedly sterilized?

Which machine agitates a mixture in a test tube to make a homogenized mixture?

Which machine rotates a mixture to separate out the different parts?

Which machine is used to measure masses?

Which piece of glassware is used to move liquid culture around an agar plate evenly?

Which piece of glassware is the most precise way to measure large amounts of liquid?

Which piece of glassware has an equal circumference from the top to the bottom and cannot be used for precise measuremnts?

Which piece of glassware is used to grow microbes in liquid culture?

Which piece of glassware is used to grow microbes in solid or semisolid agar?

Which piece of glassware/plastic is used to hold small amounts of liquid in molecular biology?

Which pipet is used to transfer the smallest amounts of liquids?

Which pipet moves a fixed volume

Which pipet is used to transfer the largest amounts of liquid?

Which piece of equipment is used to sterilize tools and glassware on the benchtop?

Which piece of equipment is used for visualizing microbes?

This is the most common type of microscope used

This type of microscope causes the light source to hit the specimen at a severely oblique angle

A benefit of this microscope is the ability to view live organisms without stains

When using the scanning objective, the objective magnification would be

When using the low-power objective, the objective magnification would be

When using the high-dry objective, the objective magnification would be

When using the oil immersion objective, the objective magnification would be

Using the provided values for multiple microscopes, what is the mean field of view in micrometers for the 4x objective?

Using the provided values for multiple microscopes, what is the mean field of view in micrometers for the 10x objective?

Using the provided values for multiple microscopes, what is the mean field of view in micrometers for the 40x objective?

Using the provided values for multiple microscopes, what is the mean field of view in micrometers for the 100x objective?

Using the scale bar, what is the size of a single E. coli cell in micrometers using the given picture?

Using the scale bar, what is the size of a P. aeruginosa cell in micrometers using the given image?

Using the scale bar, what is the size of S. epidermidis in micrometers using the provided image?

Using the scale bar, what is the size of Bacillus in micrometers using the provided image?

Using the scale bar, what is the size of a single fungi cell in micrometers?

Which type of cell wall was E. coli?

Which type of cell wall was Pseudomonas?

Which type of cell wall was Bacillus?

Which type of cell wall was Staphylococcus?

Which supergroup do Kinetoplastida, Dipomonads, Euglenazoonans, and Euglenoids belong to?

Which supergroup do Cercozoa, Radiolarians, and Foraminiferans belong to?

Which supergroup to Apicomplexans, Ciliates, and Dinoflagellates belong to?

The phylum Nematodes belongs to which kingdom?

The phylum Platyhelminthes belongs to which kingdom?

Which fungus phylum contains bread molds?

Which phylum contains the fungus that produces penicillin and brewer's yeast?

Which phylum contains the plant pathogens fungi known as smuts and rusts?

Which virus classification includes double stranded DNA?

Which virus classification includes single stranded DNA?

Which virus classification includes double stranded RNA?

Which virus classification includes positive sense single stranded RNA?

Which virus classification includes negative sense single stranded RNA?

Which virus classification includes single stranded RNA with a DNA intermediate?

Which virus classification includes double stranded DNA with an RNA intermediate?

Which type of stain sticks to the cell and gives them color?

Which type of stain does not stick to the cell, but dries around the cell boundary creating a silhouette?

Which type of stain uses both basic and acidic dyes?

Which type of stain uses acidic dyes?

Which type of stain uses a single dye?

Which type of stain uses two dyes- a primary dye and a counterstain?

Which cell structure does Gram staining react to?

What waxy material in the cell wall does acid-fast staining react to?

What cell structure gets stained when the cell is under environmental stress?

Name the two stains used in Gram staining

Name the two stains used in acid-fast staining

Name the two stains used in spore staining

Name the one stain that can be used in negative staining

Which type of cell wall is indicated by purple/blue in Gram staining?

Which type of cell wall is indicated by pink in Gram staining?

With spore staining, green indicates which structure?

With spore staining, red indicates what type of cell?

With acid-fast staining, Mycobacterium (acid-fast bacteria) will stain which color because of the presence of mycolic acid?

With acid-fast staining, non-acid-fast bacteria will stain which color?

Looking at the micrograph, is Staphylococcus aureus Gram positive or Gram negative (blue)?

Looking at the micrograph, is Pseudomonas aeruginosa Gram positive or Gram negative (pink)?

Looking at the micrograph, is Bacillus megaterium Gram positive or Gram negative (blue)?

Looking at the micrograph, is Moraxella cararrhalis Gram positive or Gram negative (pink)?

Looking at the micrograph, does the bacteria Clostridium difficile produce spores (green spores)?

Looking at the micrograph, does the bacteria Bacillus cereus produce spores (green spores)?

Looking at the micrograph, does the bacteria Staphylococcus sp. produce spores (pink, no green spores)?

Looking at the micrograph does the bacteria Bacillus megaterium produce spores (green spores)?

Looking at the micrograph, does the bacteria Mycobacterium smegmatis produce the waxy material in its cell wall (pink)?

Looking at the micrograph, does the bacteria Bacillus cereus produce the waxy material in its cell wall (blue)?

Looking at the micrograph, does the bacteria Mycobacteroum tuberculosis produce the waxy material in its cell wall (pink)?

These media are designed to grow a broad spectrum of microbes that do not have special growth requirements

These media contain complex organism substances such as growth factors that are required for the microbes to grow

These media contain substances that absorb oxygen or slow the penetration of oxygen

This type of media is used to maintain and preserve the microbe for a period of time before testing

This type of media contains one or more agents that inhibit the growth of certain microbes but not another

This type of media can grow several types of microbes but are designed to bring out visible differences among these microbes

This type of media is used to count the number of microbes in agricultural, industrial, or environmental samples

This type of media is used to test the effectiveness of microbial drugs, disinfectants and antiseptics

This type of media contain pure chemical nutrients that vary little from one source to another and have a molecular content with an exact formula

This type of media is not chemically definable by an exact formula

This type of media has large molecules such as proteins, polysaccharides, and lipids that can vary greatly in exact composition

Nutrient agar or nutrient broth is a general purpose media used for which groups?

Potato dextrose agar (PDA) is a general purpose media use for which group?

Trypticase soy agar (TSA) or broth is a general purpose media for which group?

Which of the following is commonly used to culture algae?

Which of the following is commonly used to culture paramecium?

Which of the following is commonly used to culture viruses?

The image of the colony has which type of form?

The image of the colony has which type of form?

The image of the colony has which type of form?

The image of the colony has which type of form?

The image of the colony has which type of margin?

The image of the colony has which type of margin?

The image of the colony has which type of margin?

The image of the colony has which type of margin?

The image of the colony on the agar shows which form?

The image of the colony on the agar slant shows which form?

The image of the colony has which elevation?

The image of the colony has which elevation?

The image of the colony has which elevation?

The image of the colony has which elevation?

This selective and differential media is used to isolate Staphylococcus

This selective and differential media is used to isolate enteric bacteria that hydrolyze urea

This selective and differential media is used to isolate enteric bacteria that ferment lactose

Which selective media uses high salt concentration to inhibit the growth of non-target bacteria?

Which selective media uses crystal violet and bile salts to inhibit the growth of non-target bacteria?

Which selective media uses methylene blue to inhibit the growth of non-target bacteria?

Mannitol Salt Agar is selective for which bacteria?

Eosin Methylene Blue agar is selective for which bacteria?

MacConkey's agar is selective for which bacteria?

Which media turns yellow when fermentation occurs?

Which media turns colonies of Enterobacter (Klebsiella) aerogenes pink?

Which media turns colonies of coliform bacteria red?

Cell division where genome replication is followed directly by the physical separation into two new, identical cells

Cell division where genome replication is separated by a gap phase from the physical separation into two new identical cells

In this phase, limiting factors intensify becoming detrimental to any cell growth

In this phase, the cells are adjusting to the environment

In this phase, the cells reach the maximum rate of cell division

In this phase, cells will stop growing or grow very slowly

In this phase, the majority of the cells are alive, but dead cells start to accumulate

In this phase, there are many living cells and few to none dead cells

In this phase, there are few cells present

In this phase, the dead cells take up a larger portion of the culture and little to no growth occurs

Of the following absorbance reading samples, which one has more cells present?

Of the following absorbance reading samples, which one has fewer cells present?

How many cells are present after 9 generations (starting from 1 cell) of exponential growth?

How many cells are present after 13 generations (starting from 1 cell) of exponential growth?

How many cells are present after 18 generations (starting from 1 cell) of exponential growth?

How many cells are present after 11 generations (starting from 1 cell) of exponential growth?

How many cells are present after 7 generations (starting from 1 cell) of exponential growth?

A dilution of 1mL bacteria from broth into 1mL broth or water

A dilution of 1mL bacteria from broth into 3mL broth or water

A dilution of 1mL bacteria from broth into 11mL broth or water

A dilution of 1mL bacteria from broth into 7mL broth or water

A serial dilution of 1:10 followed by 1:100 by 1:100 gives a final dilution of

A serial dilution of 1:10 followed by 1:10 followed by 1:100 gives a final dilution of

A serial dilution of 1:10 followed by 1:10 followed by 1:10 gives a final dilution of

A serial dilution of 1:6 followed by 1:8 gives a final dilution of

A serial dilution of 1:6 followed by 1:10 gives a final dilution of

A serial dilution of 1:4 followed by 1:6 gives a final dilution of

A serial dilution of 1:2 followed by 1:6 gives a final dilution of

A serial dilution of 1:6 followed by 1:12 gives a final dilution of

A dilution of 0.1mL bacteria from broth into 9.9mL

A dilution of 0.1mL bacteria from broth in 999.9mL broth or water

A dilution of 0.1mL bacteria from broth in 99.9mL broth or water

A 1:1,000,00 is which dilution factor?

A 1:100,000 is which dilution factor?

A 1:100,000,000 is which dilution factor?

A 1:10,000,000 is which dilution factor?

Microbes that require oxygen to grow but at much lower levels than 20% are

Microbes that can only grow when oxygen is not present are

Microbes that can only grow when oxygen is present are

Microbes that can grow in oxygen but do not require oxygen for energy metabolism are

Microbes that are flexible and can grow with or without oxygen are

Which bacteria have optimal growth between -5°C to 15°C?

Which bacteria have optimal growth between 45°C to 75°C?

Which bacteria have optimal growth between 25°C to 45°C?

Which bacteria have optimal growth above 75°C?

A bacterium that grows at a pH of 10 is a

A bacterium that grows at a pH of 4 is a

A bacterium that grows at a pH of 7 is a

These microbes can grow in excessive sugar concentrations

These microbes require a high concentration of sodium chloride in order to grow

These microbes can grow in moderate concentration of sodium chloride

A cell that has more solutes than the surrounding environment is

A cell that has fewer solutes than the surrounding environment

Water flows (into/out of) a cell that is hypotonic

Water flows (into/out of) a cell that is hypertonic

When the temperature increases past the optimal, the hydrogen bonding in the RNA breaks down and the proteins denature in this structure

When the temperature increases past the optimal, lipids can be destroyed affecting which structure?

Which temperature results in white colonies of Serratia?

When the temperature decreases past the optimal, lipids can freeze affecting which structure?

When the temperature decreases past the optimal, the activity of this molecule will slow down

When the temperature increases past the optimal, these denature

Which temperature results in pinkish/red colonies of Serratia?

Which temperature does Serratia grow best at?

Which temperature does E. coli grow best at?

Which temperature does GST grow best at?

Which temperature does Serratia grow the worst at?

Which temperature does E. coli grow the worst at

Which temperature does GST grow the worst at?

Which pH does E. coli grow best at?

Which pH does S. epidermidis grow best at?

Which pH does A. faecalis grow best at?

Which pH does E. coli grow the worst at?

Which pH does S. epidermidis grow the worst at?

Which pH does A. faecalis grow the worst at?