Microbiology: Pre-Lab Quizzes (1-7) Flashcards


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1

Mark all that have a prokaryotic cell type

  • Archaea
  • Bacteria

2

Mark all that have a eukaryotic cell type

  • Algaea
  • Fungi
  • Nematoda
  • Platyhelminthes
  • Protozoans

3

Mark all that are animals

  • Nematoda
  • Platyhelminthes

4

Which gene is used to molecularly identify bacteria and archaea?

16s rRNA

5

Which gene is used to molecularly identify Eukarya?

ITS

6

Which objective lens should be in position when placing a slide onto the stage or off the stage?

4x

7

Total magnification power is calculated by multiplying the objective power in use by

the ocular power (10x)

8

Which part of the microscope can be changed to make a brightfield microscope a darkfield microscope?

Condenser

9

Which part of the microscope can be changed to make a brightfield microscope a phase contrast microscope?

Diaphragm

10

When converting the field of view from mm to μm, the mm measurement is multiplied by which value?

1000

11

The simple staining method that uses one of the acidic dyes such as nigrosin or India ink

Negative Staining

12

The simple staining method that uses a single acidic or basic stain

Positive staining

13

The differential staining method that uses crystal violet and safranin

Gram staining

14

The differential staining method that uses fuchsin and methylene blue

Acid-fast staining

15

The differential staining method that uses malachite green and safranin

Spore staining

16

With negative staining, what color will bacterial colonies appear as?

White

17

With spore staining, endospores will appear as what color?

Green

18

With acid-fast staining, Mycobacterium (acid-fast bacteria) will stain which color because of the presence of mycolic acid?

Red

19

With Gram staining, Gram positive bacteria will stain as which color?

Purple/blue

20

With Gram staining, Gram negative bacteria will stain as which color?

Pink

21

Agar, a molecule derived from red algae, is an important ingredient in which type of media?

Semi-solid

22

This type of media contains one or more agents that inhibit the growth of certain microbes but not another

Selective

23

These media are designed to grow a broad spectrum of microbes that do not have special growth requirements

General purpose

24

This type of media can grow several types of microbes but are designed to bring out visible differences among those microbes

Differential

25

This media is a general purpose media used for nonfastidious microbes including many bacteria and fungi

Nutrient Agar or Broth (NA & NB)

26

This selective and differential media is used to isolate enteric bacteria that hydrolyze urea

Eosin Methylene Blue Agar (EMB)

27

This media is a general purpose media used for bacteria

Trypticase Soy Agar (TSA)

28

This media is a general purpose media used for fungi

Potato Dextrose Agar (PDA)

29

This selective and differential media is used to isolate enteric bacteria that ferment lactose

MacConkey's Agar

30

This selective and differential media is used to isolate Staphylococcus

Mannitol Salt Agar (MSA)

31

MSA is a selective medium for which bacteria because of the high salt concentration?

Staphylococcus sp.

32

MSA will turn what color when the bacterium ferments the mannitol and produces acid?

Yellow

33

EMB agar is selective for which bacteria?

Gram-negative intestinal pathogenes

34

EMB agar is selective because only bacteria that ferment this sugar produce colored colonies

Lactose

35

Colonies of E. coli on EMB agar will produce what color colonies?

Green

36

Colonies of Enterobacter aerogenes on EMB agar will produce what color colonies?

Pink

37

MacConkey's agar is selective for what group of bacteria?

Salmonella sp.

38

Colonies of coliform bacteria on MacConkey's agar will produce what color colonies?

Red

39

The ingredients of crystal violet and bile salts inhibit which group of bacteria?

Gram-positive intestinal pathogenes

40

If the nutrient agar is neither selective or differential, then the colonies of the different bacteria should look

Similar

41

Cell division for bacteria is known as

Binary fission

42

In this phase, the cells are adjusting to their environment

Lag phase

43

In this phase, the cells reach the maximum rate of cell division

Log phase

44

In this phase, the cells will stop growing or grow very slowly

Stationary phase

45

In this phase, limiting factors intensify becoming detrimental to any cell growth

Death phase

46

Generation time should be measured during which phase?

Log phase

47

A dilution of 1:10,000,000 is equivalent to 10 to which exponent?

7

48

A dilution of 1:1,000,000 is equivalent to 10 to which exponent?

6

49

When plating out a serial dilution series, which solution would have the fewest colony forming units?

1:10,000,000

50

An increase in optical density or absorbance indicates _______ microbial organisms

an increase in the number of

51

Bacteria that must grow in oxygen because their metabolism requires oxygen

Obligate aerobes

52

Bacteria that have a flexible metabolism for oxygen conditions

Facultative anaerobes

53

Bacteria that cannot grow in an oxygenated environment

Obligate anaerobes

54

Many bacteria pathogens have an optimal growth between 35°C and 40°C, these are classified as

Mesophiles

55

Which bacteria's colonies change color from white (higher temperature) to red (lower temperature)?

Serratia

56

pH refers to what?

Concentration of hydrogen ions

57

A cell that has fewer solutes than its environment

Hypotonic

58

A cell that has the same solute concentration as its environment

Isotonic

59

A cell that has more solutes than its environment

Hypertonic

60

A microbe such as Halobacterium salinarium thrives in high salt concentration environments is known as

Obligate halophile