Campbell Biology: Unit 3 AP Bio Flashcards

Which term most precisely describes the cellular process of breaking down large molecules into smaller ones?A) catalysisB) metabolism C) anabolism D) dehydration E) catabolism

Which of the following is (are) true for anabolic pathways?
A) They do not depend on enzymes.
B) They are usually highly spontaneous chemical reactions.
C) They consume energy to build up polymers from monomers.
D) They release energy as they degrade polymers to monomers.
E) They consume energy to decrease the entropy of the organism and its environment.

Which of the following is a statement of the first law of thermodynamics?
A) Energy cannot be created or destroyed.
B) The entropy of the universe is decreasing.
C) The entropy of the universe is constant.
D) Kinetic energy is stored energy that results from the specific arrangement of matter.
E) Energy cannot be transferred or transformed.

For living organisms, which of the following is an important consequence of the first law of thermodynamics?
A) The energy content of an organism is constant.
B) The organism ultimately must obtain all of the necessary energy for life from its environment.
C) The entropy of an organism decreases with time as the organism grows in complexity.
D) Organisms grow by converting energy into organic matter.
E) Life does not obey the first law of thermodynamics.

Living organisms increase in complexity as they grow, resulting in a decrease in the entropy of an organism. How does this relate to the second law of thermodynamics?
A) Living organisms do not obey the second law of thermodynamics, which states that entropy must increase with time.
B) Life obeys the second law of thermodynamics because the decrease in entropy as the organism grows is exactly balanced by an increase in the entropy of the universe.
C) Living organisms do not follow the laws of thermodynamics.
D) As a consequence of growing, organisms cause a greater increase in entropy in their environment than the decrease in entropy associated with their growth.
E) Living organisms are able to transform energy into entropy.

Whenever energy is transformed, there is always an increase in the
A) free energy of the system.
B) free energy of the universe.
C) entropy of the system.
D) entropy of the universe.
E) enthalpy of the universe.

Which of the following statements is a logical consequence of the second law of thermodynamics?
A) If the entropy of a system increases, there must be a corresponding decrease in the entropy of the universe.
B) If there is an increase in the energy of a system, there must be a corresponding decrease in the energy of the rest of the universe.
C) Every energy transfer requires activation energy from the environment.
D) Every chemical reaction must increase the total entropy of the universe.
E) Energy can be transferred or transformed, but it cannot be created or destroyed.

Which of the following statements is representative of the second law of thermodynamics?
A) Conversion of energy from one form to another is always accompanied by some gain of free energy.
B) Heat represents a form of energy that can be used by most organisms to do work.
C) Without an input of energy, organisms would tend toward decreasing entropy.
D) Cells require a constant input of energy to maintain their high level of organization.
E) Every energy transformation by a cell decreases the entropy of the universe.

Which of the following types of reactions would decrease the entropy within a cell?
A) anabolic reactions
B) hydrolysis
C) respiration
D) digestion
E) catabolic reactions

Biological evolution of life on Earth, from simple prokaryote-like cells to large, multicellar eukaryotic organisms,
A) has occurred in accordance with the laws of thermodynamics.
B) has caused an increase in the entropy of the planet.
C) has been made possible by expending Earth's energy resources.
D) has occurred in accordance with the laws of thermodynamics, by expending Earth's energy resources and causing an increase in the entropy of the planet.
E) violates the laws of thermodynamics because Earth is a closed system.

Which of the following is an example of potential rather than kinetic energy?
A) the muscle contractions of a person mowing grass
B) water rushing over Niagara Falls
C) light flashes emitted by a firefly
D) a molecule of glucose
E) the flight of an insect foraging for food

Which of the following is the smallest closed system?
A) a cell
B) an organism
C) an ecosystem
D) Earth
E) the universe

Which of the following is true of metabolism in its entirety in all organisms?
A) Metabolism depends on a constant supply of energy from food.
B) Metabolism depends on an organism's adequate hydration.
C) Metabolism uses all of an organism's resources.
D) Metabolism consists of all the energy transformation reactions in an organism.
E) Metabolism manages the increase of entropy in an organism.

The mathematical expression for the change in free energy of a system is ΔG =ΔH - TΔS. Which of the following is (are) correct?
A) ΔS is the change in enthalpy, a measure of randomness.
B) ΔH is the change in entropy, the energy available to do work.
C) ΔG is the change in free energy.
D) T is the temperature in degrees Celsius.

A system at chemical equilibrium
A) consumes energy at a steady rate.
B) releases energy at a steady rate.
C) consumes or releases energy, depending on whether it is exergonic or endergonic.
D) has zero kinetic energy.
E) can do no work.

Which of the following is true for all exergonic reactions?
A) The products have more total energy than the reactants.
B) The reaction proceeds with a net release of free energy.
C) The reaction goes only in a forward direction: all reactants will be converted to products, but no products will be converted to reactants.
D) A net input of energy from the surroundings is required for the reactions to proceed.
E) The reactions are rapid.

Chemical equilibrium is relatively rare in living cells. Which of the following could be an example of a reaction at chemical equilibrium in a cell?
A) a reaction in which the free energy at equilibrium is higher than the energy content at any point away from equilibrium
B) a chemical reaction in which the entropy change in the reaction is just balanced by an opposite entropy change in the cell's surroundings
C) an endergonic reaction in an active metabolic pathway where the energy for that reaction is supplied only by heat from the environment
D) a chemical reaction in which both the reactants and products are not being produced or used in any active metabolic pathway
E) no possibility of having chemical equilibrium in any living cell

Which of the following shows the correct changes in thermodynamic properties for a chemical reaction in which amino acids are linked to form a protein?
A) +ΔH, +ΔS, +ΔG
B) +ΔH, -ΔS, -ΔG
C) +ΔH, -ΔS, +ΔG
D) -ΔH, -ΔS, +ΔG
E) -ΔH, +ΔS, +ΔG

When glucose monomers are joined together by glycosidic linkages to form a cellulose polymer, the changes in free energy, total energy, and entropy are as follows:
A) +ΔG, +ΔH, +ΔS.
B) +ΔG, +ΔH, -ΔS.
C) +ΔG, -ΔH, -ΔS.
D) -ΔG, +ΔH, +ΔS.
E) -ΔG, -ΔH, -ΔS.

A chemical reaction that has a positive ΔG is correctly described as
A) endergonic.
B) endothermic.
C) enthalpic.
D) spontaneous.
E) exothermic.

Which of the following best describes enthalpy (H)?
A) the total kinetic energy of a system
B) the heat content of a chemical system
C) the system's entropy
D) the cell's energy equilibrium
E) the condition of a cell that is not able to react

For the hydrolysis of ATP to ADP + Pi, the free energy change is -7.3 kcal/mol under standard conditions (1 M concentration of both reactants and products). In the cellular environment, however, the free energy change is about -13 kcal/mol. What can we conclude about the free energy change for the formation of ATP from ADP and Pi under cellular conditions?
A) It is +7.3 kcal/mol.
B) It is less than +7.3 kcal/mol.
C) It is about +13 kcal/mol.
D) It is greater than +13 kcal/mol.
E) The information given is insufficient to deduce the free energy change.

Why is ATP an important molecule in metabolism?
A) Its hydrolysis provides an input of free energy for exergonic reactions.
B) It provides energy coupling between exergonic and endergonic reactions.
C) Its terminal phosphate group contains a strong covalent bond that, when hydrolyzed, releases free energy.
D) Its terminal phosphate bond has higher energy than the other two.
E) It is one of the four building blocks for DNA synthesis.

When 10,000 molecules of ATP are hydrolyzed to ADP and Pi in a test tube, about twice as much heat is liberated as when a cell hydrolyzes the same amount of ATP. Which of the following is the best explanation for this observation?
A) Cells are open systems, but a test tube is a closed system.
B) Cells are less efficient at heat production than nonliving systems.
C) The hydrolysis of ATP in a cell produces different chemical products than does the reaction in a test tube.
D) The reaction in cells must be catalyzed by enzymes, but the reaction in a test tube does not need enzymes.
E) Reactant and product concentrations in the test tube are different from those in the cell.

Which of the following is most similar in structure to ATP?
A) a pentose sugar
B) a DNA nucleotide
C) an RNA nucleotide
D) an amino acid with three phosphate groups attached
E) a phospholipid

Which of the following statements is true concerning catabolic pathways?
A) They combine molecules into more energy-rich molecules.
B) They supply energy, primarily in the form of ATP, for the cell's work.
C) They are endergonic.
D) They are spontaneous and do not need enzyme catalysis.
E) They build up complex molecules such as protein from simpler compounds.

When chemical, transport, or mechanical work is done by an organism, what happens to the heat generated?
A) It is used to power yet more cellular work.
B) It is used to store energy as more ATP.
C) It is used to generate ADP from nucleotide precursors.
D) It is lost to the environment.
E) It is transported to specific organs such as the brain.

When ATP releases some energy, it also releases inorganic phosphate. What purpose does this serve (if any) in the cell?
A) The phosphate is released as an excretory waste.
B) The phosphate can only be used to regenerate more ATP.
C) The phosphate can be added to water and excreted as a liquid.
D) The phosphate may be incorporated into any molecule that contains phosphate.
E) It enters the nucleus to affect gene expression.

A number of systems for pumping ions across membranes are powered by ATP. Such ATP-powered pumps are often called ATPases although they don't often hydrolyze ATP unless they are simultaneously transporting ions. Because small increases in calcium ions in the cytosol can trigger a number of different intracellular reactions, cells keep the cytosolic calcium concentration quite low under normal conditions, using ATP-powered calcium pumps. For example, muscle cells transport calcium from the cytosol into the membranous system called the sarcoplasmic reticulum (SR). If a resting muscle cell's cytosol has a free calcium ion concentration of 10⁻⁷ while the concentration in the SR is 10⁻², then how is the ATPase acting?
A) ATPase activity must be powering an inflow of calcium from the outside of the cell into the SR.
B) ATPase activity must be transferring Pi to the SR to enable this to occur.
C) ATPase activity must be pumping calcium from the cytosol to the SR against the concentration gradient.
D) ATPase activity must be opening a channel for the calcium ions to diffuse back into the SR along the concentration gradient.
E) ATPase activity must be routing calcium ions from the SR to the cytosol, and then to the cell's environment.

What is the difference (if any) between the structure of ATP and the structure of the precursor of the A nucleotide in RNA?
A) The sugar molecule is different.
B) The nitrogen-containing base is different.
C) The number of phosphates is three instead of one.
D) The number of phosphates is three instead of two.
E) There is no difference.

Which of the following statements is true about enzyme-catalyzed reactions?
A) The reaction is faster than the same reaction in the absence of the enzyme.
B) The free energy change of the reaction is opposite from the reaction that occurs in the absence of the enzyme.
C) The reaction always goes in the direction toward chemical equilibrium.
D) Enzyme-catalyzed reactions require energy to activate the enzyme.
E) Enzyme-catalyzed reactions release more free energy than noncatalyzed reactions.

Reactants capable of interacting to form products in a chemical reaction must first overcome a thermodynamic barrier known as the reaction's
A) entropy.
B) activation energy.
C) endothermic level.
D) equilibrium point.
E) free-energy content.

A solution of starch at room temperature does not readily decompose to form a solution of simple sugars because
A) the starch solution has less free energy than the sugar solution.
B) the hydrolysis of starch to sugar is endergonic.
C) the activation energy barrier for this reaction cannot be surmounted.
D) starch cannot be hydrolyzed in the presence of so much water.
E) starch hydrolysis is nonspontaneous.

Which of the following statements regarding enzymes is true?
A) Enzymes increase the rate of a reaction by making the reaction more exergonic.
B) Enzymes increase the rate of a reaction by lowering the activation energy barrier.
C) Enzymes increase the rate of a reaction by reducing the rate of reverse reactions.
D) Enzymes change the equilibrium point of the reactions they catalyze.
E) Enzymes make the rate of a reaction independent of substrate concentrations.

During a laboratory experiment, you discover that an enzyme-catalyzed reaction has a ∆G of -20 kcal/mol. If you double the amount of enzyme in the reaction, what will be the ∆G for the new reaction?
A) -40 kcal/mol
B) -20 kcal/mol
C) 0 kcal/mol
D) +20 kcal/mol
E) +40 kcal/mol

The active site of an enzyme is the region that
A) binds allosteric regulators of the enzyme.
B) is involved in the catalytic reaction of the enzyme.
C) binds noncompetitive inhibitors of the enzyme.
D) is inhibited by the presence of a coenzyme or a cofactor

According to the induced fit hypothesis of enzyme catalysis, which of the following is correct?
A) The binding of the substrate depends on the shape of the active site.
B) Some enzymes change their structure when activators bind to the enzyme.
C) A competitive inhibitor can outcompete the substrate for the active site.
D) The binding of the substrate changes the shape of the enzyme's active site.
E) The active site creates a microenvironment ideal for the reaction.

Mutations that result in single amino acid substitutions in an enzyme
A) can have no effect on the activity or properties of the enzyme.
B) will almost always destroy the activity of the enzyme.
C) will often cause a change in the substrate specificity of the enzyme.
D) may affect the physicochemical properties of the enzyme such as its optimal temperature and pH.
E) may, in rare cases, cause the enzyme to run reactions in reverse

Increasing the substrate concentration in an enzymatic reaction could overcome which of the following?
A) denaturization of the enzyme
B) allosteric inhibition
C) competitive inhibition
D) saturation of the enzyme activity
E) insufficient cofactors

Which of the following is true of enzymes?
A) Nonprotein cofactors alter the substrate specificity of enzymes.
B) Enzyme function is increased if the 3-D structure or conformation of an enzyme is altered.
C) Enzyme function is independent of physical and chemical environmental factors such as pH and temperature.
D) Enzymes increase the rate of chemical reaction by lowering activation energy barriers.
E) Enzymes increase the rate of chemical reaction by providing activation energy to the substrate.

Zinc, an essential trace element for most organisms, is present in the active site of the enzyme carboxypeptidase. The zinc most likely functions as a(n)
A) competitive inhibitor of the enzyme.
B) noncompetitive inhibitor of the enzyme.
C) allosteric activator of the enzyme.
D) cofactor necessary for enzyme activity.
E) coenzyme derived from a vitamin.

In order to attach a particular amino acid to the tRNA molecule that will transport it, an enzyme, an aminoacyl-tRNA synthetase, is required, along with ATP. Initially, the enzyme has an active site for ATP and another for the amino acid, but it is not able to attach the tRNA. What must occur in order for the final attachment to occur?
A) The ATP must first have to attach to the tRNA.
B) The binding of the first two molecules must cause a 3-D change that opens another active site on the enzyme.
C) The ATP must be hydrolyzed to allow the amino acid to bind to the synthetase.
D) The tRNA molecule must have to alter its shape in order to be able to fit into the active site with the other two molecules.
E) The 3' end of the tRNA must have to be cleaved before it can have an attached amino acid.

Some of the drugs used to treat HIV patients are competitive inhibitors of the HIV reverse transcriptase enzyme. Unfortunately, the high mutation rate of HIV means that the virus rapidly acquires mutations with amino acid changes that make them resistant to these competitive inhibitors. Where in the reverse transcriptase enzyme would such amino acid changes most likely occur in drug-resistant viruses?
A) in or near the active site
B) at an allosteric site
C) at a cofactor binding site
D) in regions of the protein that determine packaging into the virus capsid
E) such mutations could occur anywhere with equal probability

Protein kinases are enzymes that transfer the terminal phosphate from ATP to an amino acid residue on the target protein. Many are located on the plasma membrane as integral membrane proteins or peripheral membrane proteins. What purpose may be served by their plasma membrane localization?
A) ATP is more abundant near the plasma membrane.
B) They can more readily encounter and phosphorylate other membrane proteins.
C) Membrane localization lowers the activation energy of the phosphorylation reaction.
D) They flip back and forth across the membrane to access target proteins on either side.
E) They require phospholipids as a cofactor.

When you have a severe fever, what grave consequence may occur if the fever is not controlled?
A) destruction of your enzymes' primary structure
B) removal of amine groups from your proteins
C) change in the tertiary structure of your enzymes
D) removal of the amino acids in active sites of your enzymes
E) binding of your enzymes to inappropriate substrates

How does a noncompetitive inhibitor decrease the rate of an enzyme reaction?
A) by binding at the active site of the enzyme
B) by changing the shape of the enzyme's active site
C) by changing the free energy change of the reaction
D) by acting as a coenzyme for the reaction
E) by decreasing the activation energy of the reaction

The mechanism in which the end product of a metabolic pathway inhibits an earlier step in the pathway is most precisely described as
A) metabolic inhibition.
B) feedback inhibition.
C) allosteric inhibition.
D) noncooperative inhibition.
E) reversible inhibition.

Which of the following statements describes enzyme cooperativity?
A) A multienzyme complex contains all the enzymes of a metabolic pathway.
B) A product of a pathway serves as a competitive inhibitor of an early enzyme in the pathway.
C) A substrate molecule bound to an active site of one subunit promotes substrate binding to the active site of other subunits.
D) Several substrate molecules can be catalyzed by the same enzyme.
E) A substrate binds to an active site and inhibits cooperation between enzymes in a pathway.

Allosteric enzyme regulation is usually associated with
A) lack of cooperativity.
B) feedback inhibition.
C) activating activity.
D) an enzyme with more than one subunit.
E) the need for cofactors.

Which of the following is an example of cooperativity?
A) the binding of an end product of a metabolic pathway to the first enzyme that acts in the pathway
B) one enzyme in a metabolic pathway passing its product to act as a substrate for the next enzyme in the pathway
C) a molecule binding at one unit of a tetramer, allowing faster binding at each of the other three
D) the effect of increasing temperature on the rate of an enzymatic reaction
E) binding of an ATP molecule along with one of the substrate molecules in an active site

Protein kinases are enzymes that catalyze phosphorylation of target proteins at specific sites, whereas protein phosphatases catalyze removal of phosphate(s) from phosphorylated proteins. Phosphorylation and dephosphorylation can function as an on-off switch for a protein's activity, most likely through
A) the change in a protein's charge leading to a conformational change.
B) the change in a protein's charge leading to cleavage.
C) a change in the optimal pH at which a reaction will occur.
D) a change in the optimal temperature at which a reaction will occur.
E) the excision of one or more peptides.

Besides turning enzymes on or off, what other means does a cell use to control enzymatic activity?
A) cessation of cellular protein synthesis
B) localization of enzymes into specific organelles or membranes
C) exporting enzymes out of the cell
D) connecting enzymes into large aggregates
E) hydrophobic interactions

An important group of peripheral membrane proteins are enzymes such as the phospholipases that cleave the head groups of phospholipids. What properties must these enzymes exhibit?
A) resistance to degradation
B) independence from cofactor interaction
C) water solubility
D) lipid solubility
E) membrane-spanning domains

In experimental tests of enzyme evolution, where a gene encoding an enzyme is subjected to multiple cycles of random mutagenesis and selection for altered substrate specificity, the resulting enzyme had multiple amino acid changes associated with altered substrate specificity. Where in the enzyme were these amino acid changes located?
A) only in the active site
B) only in the active site or near the active site
C) in or near the active site and at surface sites away from the active site
D) only at surface sites away from the active site
E) only in the hydrophobic interior of the folded protein

How might an amino acid change at a site distant from the active site of the enzyme alter the enzyme's substrate specificity?
A) by changing the enzyme's stability
B) by changing the enzyme's location in the cell
C) by changing the shape of the protein
D) by changing the enzyme's pH optimum
E) an amino acid change away from the active site cannot alter the enzyme's substrate specificity

For the enzyme-catalyzed reaction shown in the figure, which of these treatments will cause the greatest increase in the rate of the reaction, if the initial reactant concentration is 1.0 micromolar?
A) doubling the activation energy needed
B) cooling the reaction by 10°C
C) doubling the concentration of the reactants to 2.0 micromolar
D) doubling the enzyme concentration
E) increasing the concentration of reactants to 10.0 micromolar, while reducing the concentration of enzyme by 1/2

In the figure, why does the reaction rate plateau at higher reactant concentrations?
A) Feedback inhibition by product occurs at high reactant concentrations.
B) Most enzyme molecules are occupied by substrate at high reactant concentrations.
C) The reaction nears equilibrium at high reactant concentrations.
D) The activation energy for the reaction increases with reactant concentration.
E) The rate of the reverse reaction increases with reactant concentration.

Which curve(s) on the graphs may represent the temperature and pH profiles of an enzyme taken from a bacterium that lives in a mildly alkaline hot springs at temperatures of 70°C or higher?
A) curves 1 and 5
B) curves 2 and 4
C) curves 2 and 5
D) curves 3 and 4
E) curves 3 and 5

Which temperature and pH profile curves on the graphs were most likely generated from analysis of an enzyme from a human stomach where conditions are strongly acid?
A) curves 1 and 4
B) curves 1 and 5
C) curves 2 and 4
D) curves 2 and 5
E) curves 3 and 4

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following terms best describes the forward reaction in Figure 8.1?
A) endergonic, ∆G > 0
B) exergonic, ∆G < 0
C) endergonic, ∆G < 0
D) exergonic, ∆G > 0
E) chemical equilibrium, ∆G = 0

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following represents the ΔG of the reaction in Figure 8.1?
A) A
B) B
C) C
D) D
E) E

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following in Figure 8.1 would be the same in either an enzyme-catalyzed or a noncatalyzed reaction?
A) A
B) B
C) C
D) D
E) E

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following represents the activation energy needed for the enzyme-catalyzed reverse reaction, C + D → A + B, in Figure 8.1?
A) A
B) B
C) C
D) D
E) E

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following represents the difference between the free-energy content of the reaction and the free-energy content of the products in Figure 8.1?
A) A
B) B
C) C
D) D
E) E

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following represents the activation energy required for the enzyme-catalyzed reaction in Figure 8.1?
A) A
B) B
C) C
D) D
E) E

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following represents the activation energy required for a noncatalyzed reaction in Figure 8.1?
A) A
B) B
C) C
D) D
E) E

This question is based on the reaction A + B ↔ C + D shown in the figure.

Which of the following represents the activation energy needed for the noncatalyzed reverse reaction, C + D → A + B, in Figure 8.1?
A) A
B) B
C) C
D) D
E) E

This question is based on the reaction A + B ↔ C + D shown in the figure.

Assume that the reaction in Figure 8.1 has a ΔG of -5.6 kcal/mol. Which of the following would be true?
A) The reaction could be coupled to power an endergonic reaction with a ΔG of +6.2 kcal/mol.
B) The reaction could be coupled to power an exergonic reaction with a ΔG of +8.8 kcal/mol.
C) The reaction would result in a decrease in entropy (S) and an increase in the total energy content (H) of the system.
D) The reaction would result in an increase in entropy (S) and a decrease in the total energy content (H) of the system.
E) The reaction would result in products (C + D) with a greater free-energy content than in the initial reactants (A + B).

Which of the following is the most correct interpretation of the figure?
A) Inorganic phosphate is created from organic phosphate.
B) Energy from catabolism can be used directly for performing cellular work.
C) ADP + Pi are a set of molecules that store energy for catabolism.
D) ATP is a molecule that acts as an intermediary to store energy for cellular work.
E) Pi acts as a shuttle molecule to move energy from ATP to ADP.

How do cells use the ATP cycle shown in the figure?
A) Cells use the cycle to recycle ADP and phosphate.
B) Cells use the cycle to recycle energy released by ATP hydrolysis.
C) Cells use the cycle to recycle ADP, phosphate, and the energy released by ATP hydrolysis.
D) Cells use the cycle to generate or consume water molecules as needed.
E) Cells use the cycle primarily to generate heat.

Succinate dehydrogenase catalyzes the conversion of succinate to fumarate. The reaction is inhibited by malonic acid, which resembles succinate but cannot be acted upon by succinate dehydrogenase. Increasing the ratio of succinate to malonic acid reduces the inhibitory effect of malonic acid.

Based on this information, which of the following is correct?
A) Succinate dehydrogenase is the enzyme, and fumarate is the substrate.
B) Succinate dehydrogenase is the enzyme, and malonic acid is the substrate.
C) Succinate is the substrate, and fumarate is the product.
D) Fumarate is the product, and malonic acid is a noncompetitive inhibitor.
E) Malonic acid is the product, and fumarate is a competitive inhibitor.

Succinate dehydrogenase catalyzes the conversion of succinate to fumarate. The reaction is inhibited by malonic acid, which resembles succinate but cannot be acted upon by succinate dehydrogenase. Increasing the ratio of succinate to malonic acid reduces the inhibitory effect of malonic acid.

What is malonic acid's role with respect to succinate dehydrogenase?
A) It is a competitive inhibitor.
B) It blocks the binding of fumarate.
C) It is a noncompetitive inhibitor.
D) It is able to bind to succinate.
E) It is an allosteric regulator.

A series of enzymes catalyze the reaction X → Y → Z → A. Product A binds to the enzyme that converts X to Y at a position remote from its active site. This binding decreases the activity of the enzyme.

What is substance X?
A) a coenzyme
B) an allosteric inhibitor
C) a substrate
D) an intermediate
E) the product

A series of enzymes catalyze the reaction X → Y → Z → A. Product A binds to the enzyme that converts X to Y at a position remote from its active site. This binding decreases the activity of the enzyme.

With respect to the enzyme that converts X to Y, substance A functions as
A) a coenzyme.
B) an allosteric inhibitor.
C) the substrate.
D) an intermediate.
E) a competitive inhibitor.

Choose the pair of terms that correctly completes this sentence: Catabolism is to anabolism as ________ is to ________.
A) exergonic; spontaneous
B) exergonic; endergonic
C) free energy; entropy
D) work; energy
E) entropy; enthalpy

Most cells cannot harness heat to perform work because
A) heat is not a form of energy.
B) cells do not have much heat; they are relatively cool.
C) temperature is usually uniform throughout a cell.
D) heat can never be used to do work.
E) heat must remain constant during work.

Which of the following metabolic processes can occur without a net influx of energy from some other process?
A) ADP + Pi → ATP + H₂O
B) C₆H₁₂O₆ + 6 O₂ → 6 CO₂ + 6 H₂O
C) 6 CO₂ + 6 H₂O → C₆H₁₂O₆ + 6 O₂
D) amino acids → protein
E) glucose + fructose → sucrose

If an enzyme in solution is saturated with substrate, the most effective way to obtain a faster yield of products is to
A) add more of the enzyme.
B) heat the solution to 90°C.
C) add more substrate.
D) add an allosteric inhibitor.
E) add a noncompetitive inhibitor.

Some bacteria are metabolically active in hot springs because
A) they are able to maintain a lower internal temperature.
B) high temperatures make catalysis unnecessary.
C) their enzymes have high optimal temperatures.
D) their enzymes are completely insensitive to temperature.
E) they use molecules other than proteins or RNAs as their main catalysts.

If an enzyme is added to a solution where its substrate and product are in equilibrium, what will occur?
A) Additional product will be formed.
B) Additional substrate will be formed.
C) The reaction will change from endergonic to exergonic.
D) The free energy of the system will change.
E) Nothing; the reaction will stay at equilibrium.

What is the term for metabolic pathways that release stored energy by breaking down complex molecules?
A) anabolic pathways
B) catabolic pathways
C) fermentation pathways
D) thermodynamic pathways
E) bioenergetic pathways

The molecule that functions as the reducing agent (electron donor) in a redox or oxidation-reduction reaction
A) gains electrons and gains potential energy.
B) loses electrons and loses potential energy.
C) gains electrons and loses potential energy.
D) loses electrons and gains potential energy.
E) neither gains nor loses electrons, but gains or loses potential energy.

When electrons move closer to a more electronegative atom, what happens?
A) The more electronegative atom is reduced, and energy is released.
B) The more electronegative atom is reduced, and energy is consumed.
C) The more electronegative atom is oxidized, and energy is consumed.
D) The more electronegative atom is oxidized, and energy is released.
E) The more electronegative atom is reduced, and entropy decreases.

Why does the oxidation of organic compounds by molecular oxygen to produce CO₂ and water release free energy?
A) The covalent bonds in organic molecules and molecular oxygen have more kinetic energy than the covalent bonds in water and carbon dioxide.
B) Electrons are being moved from atoms that have a lower affinity for electrons (such as C) to atoms with a higher affinity for electrons (such as O).
C) The oxidation of organic compounds can be used to make ATP.
D) The electrons have a higher potential energy when associated with water and CO₂ than they do in organic compounds.
E) The covalent bond in O₂ is unstable and easily broken by electrons from organic molecules.

Which of the following statements describes the results of this reaction?
C₆H₁₂O₆ + 6 O₂ → 6 CO₂ + 6 H₂O + Energy
A) C₆H₁₂O₆ is oxidized and O₂ is reduced.
B) O₂ is oxidized and H₂O is reduced.
C) CO₂ is reduced and O₂ is oxidized.
D) C₆H₁₂O₆ is reduced and CO₂ is oxidized.
E) O₂ is reduced and CO₂ is oxidized.

When a glucose molecule loses a hydrogen atom as the result of an oxidation-reduction reaction, the molecule becomes
A) hydrolyzed.
B) hydrogenated.
C) oxidized.
D) reduced.
E) an oxidizing agent.

When a molecule of NAD⁺ (nicotinamide adenine dinucleotide) gains a hydrogen atom (not a proton), the molecule becomes
A) dehydrogenated.
B) oxidized.
C) reduced.
D) redoxed.
E) hydrolyzed.

Which of the following statements describes NAD⁺?
A) NAD⁺ is reduced to NADH during glycolysis, pyruvate oxidation, and the citric acid cycle.
B) NAD⁺ has more chemical energy than NADH.
C) NAD⁺ is oxidized by the action of hydrogenases.
D) NAD⁺ can donate electrons for use in oxidative phosphorylation.
E) In the absence of NAD⁺, glycolysis can still function.

Where does glycolysis take place in eukaryotic cells?
A) mitochondrial matrix
B) mitochondrial outer membrane
C) mitochondrial inner membrane
D) mitochondrial intermembrane space
E) cytosol

The ATP made during glycolysis is generated by
A) substrate-level phosphorylation.
B) electron transport.
C) photophosphorylation.
D) chemiosmosis.
E) oxidation of NADH to NAD⁺.

The oxygen consumed during cellular respiration is involved directly in which process or event?
A) glycolysis
B) accepting electrons at the end of the electron transport chain
C) the citric acid cycle
D) the oxidation of pyruvate to acetyl CoA
E) the phosphorylation of ADP to form ATP

Which process in eukaryotic cells will proceed normally whether oxygen (O₂) is present or absent?
A) electron transport
B) glycolysis
C) the citric acid cycle
D) oxidative phosphorylation
E) chemiosmosis

An electron loses potential energy when it
A) shifts to a less electronegative atom.
B) shifts to a more electronegative atom.
C) increases its kinetic energy.
D) increases its activity as an oxidizing agent.
E) moves further away from the nucleus of the atom.

Why are carbohydrates and fats considered high energy foods?
A) They have a lot of oxygen atoms.
B) They have no nitrogen in their makeup.
C) They can have very long carbon skeletons.
D) They have a lot of electrons associated with hydrogen.
E) They are easily reduced.

Substrate-level phosphorylation accounts for approximately what percentage of the ATP formed by the reactions of glycolysis?
A) 0%
B) 2%
C) 10%
D) 38%
E) 100%

During glycolysis, when each molecule of glucose is catabolized to two molecules of pyruvate, most of the potential energy contained in glucose is
A) transferred to ADP, forming ATP.
B) transferred directly to ATP.
C) retained in the two pyruvates.
D) stored in the NADH produced.
E) used to phosphorylate fructose to form fructose 6-phosphate.

In addition to ATP, what are the end products of glycolysis?
A) CO₂ and H₂O
B) CO₂ and pyruvate
C) NADH and pyruvate
D) CO₂ and NADH
E) H₂O, FADH₂, and citrate

The free energy for the oxidation of glucose to CO₂ and water is -686 kcal/mol and the free energy for the reduction of NAD⁺ to NADH is +53 kcal/mol. Why are only two molecules of NADH formed during glycolysis when it appears that as many as a dozen could be formed?
A) Most of the free energy available from the oxidation of glucose is used in the production of ATP in glycolysis.
B) Glycolysis is a very inefficient reaction, with much of the energy of glucose released as heat.
C) Most of the free energy available from the oxidation of glucose remains in pyruvate, one of the products of glycolysis.
D) There is no CO₂ or water produced as products of glycolysis.
E) Glycolysis consists of many enzymatic reactions, each of which extracts some energy from the glucose molecule.

Starting with one molecule of glucose, the energy-containing products of glycolysis are
A) 2 NAD⁺, 2 pyruvate, and 2 ATP.
B) 2 NADH, 2 pyruvate, and 2 ATP.
C) 2 FADH₂, 2 pyruvate, and 4 ATP.
D) 6 CO₂, 2 ATP, and 2 pyruvate.
E) 6 CO₂, 30 ATP, and 2 pyruvate.

In glycolysis, for each molecule of glucose oxidized to pyruvate
A) two molecules of ATP are used and two molecules of ATP are produced.
B) two molecules of ATP are used and four molecules of ATP are produced.
C) four molecules of ATP are used and two molecules of ATP are produced.
D) two molecules of ATP are used and six molecules of ATP are produced.
E) six molecules of ATP are used and six molecules of ATP are produced.

A molecule that is phosphorylated
A) has been reduced as a result of a redox reaction involving the loss of an inorganic phosphate.
B) has a decreased chemical reactivity; it is less likely to provide energy for cellular work.
C) has been oxidized as a result of a redox reaction involving the gain of an inorganic phosphate.
D) has an increased chemical potential energy; it is primed to do cellular work.
E) has less energy than before its phosphorylation and therefore less energy for cellular work.

Which kind of metabolic poison would most directly interfere with glycolysis?
A) an agent that reacts with oxygen and depletes its concentration in the cell
B) an agent that binds to pyruvate and inactivates it
C) an agent that closely mimics the structure of glucose but is not metabolized
D) an agent that reacts with NADH and oxidizes it to NAD⁺
E) an agent that blocks the passage of electrons along the electron transport chain

Why is glycolysis described as having an investment phase and a payoff phase?
A) It both splits molecules and assembles molecules.
B) It attaches and detaches phosphate groups.
C) It uses glucose and generates pyruvate.
D) It shifts molecules from cytosol to mitochondrion.
E) It uses stored ATP and then forms a net increase in ATP.

The transport of pyruvate into mitochondria depends on the proton-motive force across the inner mitochondrial membrane. How does pyruvate enter the mitochondrion?
A) active transport
B) diffusion
C) facilitated diffusion
D) through a channel
E) through a pore

Which of the following intermediary metabolites enters the citric acid cycle and is formed, in part, by the removal of a carbon (CO₂) from one molecule of pyruvate?
A) lactate
B) glyceraldehydes-3-phosphate
C) oxaloacetate
D) acetyl CoA
E) citrate

During cellular respiration, acetyl CoA accumulates in which location?
A) cytosol
B) mitochondrial outer membrane
C) mitochondrial inner membrane
D) mitochondrial intermembrane space
E) mitochondrial matrix

How many carbon atoms are fed into the citric acid cycle as a result of the oxidation of one molecule of pyruvate?
A) two
B) four
C) six
D) eight
E) ten

Carbon dioxide (CO₂) is released during which of the following stages of cellular respiration?
A) glycolysis and the oxidation of pyruvate to acetyl CoA
B) oxidation of pyruvate to acetyl CoA and the citric acid cycle
C) the citric acid cycle and oxidative phosphorylation
D) oxidative phosphorylation and fermentation
E) fermentation and glycolysis

A young animal has never had much energy. He is brought to a veterinarian for help and is sent to the animal hospital for some tests. There they discover his mitochondria can use only fatty acids and amino acids for respiration, and his cells produce more lactate than normal. Of the following, which is the best explanation of his condition?
A) His mitochondria lack the transport protein that moves pyruvate across the outer mitochondrial membrane.
B) His cells cannot move NADH from glycolysis into the mitochondria.
C) His cells contain something that inhibits oxygen use in his mitochondria.
D) His cells lack the enzyme in glycolysis that forms pyruvate.
E) His cells have a defective electron transport chain, so glucose goes to lactate instead of to acetyl CoA.

During aerobic respiration, electrons travel downhill in which sequence?
A) food → citric acid cycle → ATP → NAD⁺
B) food → NADH → electron transport chain → oxygen
C) glucose → pyruvate → ATP → oxygen
D) glucose → ATP → electron transport chain → NADH
E) food → glycolysis → citric acid cycle → NADH → ATP

What fraction of the carbon dioxide exhaled by animals is generated by the reactions of the citric acid cycle, if glucose is the sole energy source?
A) 1/6
B) 1/3
C) 1/2
D) 2/3
E) 100/100

Where are the proteins of the electron transport chain located?
A) cytosol
B) mitochondrial outer membrane
C) mitochondrial inner membrane
D) mitochondrial intermembrane space
E) mitochondrial matrix

In cellular respiration, the energy for most ATP synthesis is supplied by
A) high energy phosphate bonds in organic molecules.
B) a proton gradient across a membrane.
C) converting oxygen to ATP.
D) transferring electrons from organic molecules to pyruvate.
E) generating carbon dioxide and oxygen in the electron transport chain.

During aerobic respiration, which of the following directly donates electrons to the electron transport chain at the lowest energy level?
A) NAD+
B) NADH
C) ATP
D) ADP + Pi
E) FADH2

The primary role of oxygen in cellular respiration is to
A) yield energy in the form of ATP as it is passed down the respiratory chain.
B) act as an acceptor for electrons and hydrogen, forming water.
C) combine with carbon, forming CO₂.
D) combine with lactate, forming pyruvate.
E) catalyze the reactions of glycolysis.

Inside an active mitochondrion, most electrons follow which pathway?
A) glycolysis → NADH → oxidative phosphorylation → ATP → oxygen
B) citric acid cycle → FADH₂ → electron transport chain → ATP
C) electron transport chain → citric acid cycle → ATP → oxygen
D) pyruvate → citric acid cycle → ATP → NADH → oxygen
E) citric acid cycle → NADH → electron transport chain → oxygen

During aerobic respiration, H₂O is formed. Where does the oxygen atom for the formation of the water come from?
A) carbon dioxide (CO₂)
B) glucose (C₆H₁₂O₆)
C) molecular oxygen (O₂)
D) pyruvate (C₃H₃O₃-)
E) lactate (C₃H₅O₃-)

In chemiosmotic phosphorylation, what is the most direct source of energy that is used to convert ADP + Pi to ATP?
A) energy released as electrons flow through the electron transport system
B) energy released from substrate-level phosphorylation
C) energy released from movement of protons through ATP synthase, against the electrochemical gradient
D) energy released from movement of protons through ATP synthase, down the electrochemical gradient
E) No external source of energy is required because the reaction is exergonic.

Energy released by the electron transport chain is used to pump H⁺ into which location in eukaryotic cells?
A) cytosol
B) mitochondrial outer membrane
C) mitochondrial inner membrane
D) mitochondrial intermembrane space
E) mitochondrial matrix

The direct energy source that drives ATP synthesis during respiratory oxidative phosphorylation in eukaryotic cells is
A) oxidation of glucose to CO₂ and water.
B) the thermodynamically favorable flow of electrons from NADH to the mitochondrial electron transport carriers.
C) the final transfer of electrons to oxygen.
D) the proton-motive force across the inner mitochondrial membrane.
E) the thermodynamically favorable transfer of phosphate from glycolysis and the citric acid cycle intermediate molecules of ADP.

When hydrogen ions are pumped from the mitochondrial matrix across the inner membrane and into the intermembrane space, the result is the
A) formation of ATP.
B) reduction of NAD⁺.
C) restoration of the Na⁺/K⁺ balance across the membrane.
D) creation of a proton-motive force.
E) lowering of pH in the mitochondrial matrix.

Where is ATP synthase located in the mitochondrion?
A) cytosol
B) electron transport chain
C) outer membrane
D) inner membrane
E) mitochondrial matrix

It is possible to prepare vesicles from portions of the inner mitochondrial membrane. Which one of the following processes could still be carried on by this isolated inner membrane?
A) the citric acid cycle
B) oxidative phosphorylation
C) glycolysis and fermentation
D) reduction of NAD⁺
E) both the citric acid cycle and oxidative phosphorylation

How many oxygen molecules (O₂) are required each time a molecule of glucose (C₆H₁₂O₆) is completely oxidized to carbon dioxide and water via aerobic respiration,?
A) 1
B) 3
C) 6
D) 12
E) 30

Which of the following produces the most ATP when glucose (C₆H₁₂O₆) is completely oxidized to carbon dioxide (CO₂) and water?
A) glycolysis
B) fermentation
C) oxidation of pyruvate to acetyl CoA
D) citric acid cycle
E) oxidative phosphorylation (chemiosmosis)

Approximately how many molecules of ATP are produced from the complete oxidation of two molecules of glucose (C₆H₁₂O₆) in aerobic cellular respiration?
A) 2
B) 4
C) 15
D) 30-32
E) 60-64

The synthesis of ATP by oxidative phosphorylation, using the energy released by movement of protons across the membrane down their electrochemical gradient, is an example of
A) active transport.
B) an endergonic reaction coupled to an exergonic reaction.
C) a reaction with a positive ΔG .
D) osmosis.
E) allosteric regulation.

Chemiosmotic ATP synthesis (oxidative phosphorylation) occurs in
A) all cells, but only in the presence of oxygen.
B) only eukaryotic cells, in the presence of oxygen.
C) only in mitochondria, using either oxygen or other electron acceptors.
D) all respiring cells, both prokaryotic and eukaryotic, using either oxygen or other electron acceptors.
E) all cells, in the absence of respiration.

If a cell is able to synthesize 30 ATP molecules for each molecule of glucose completely oxidized by carbon dioxide and water, how many ATP molecules can the cell synthesize for each molecule of pyruvate oxidized to carbon dioxide and water?
A) 0
B) 1
C) 12
D) 14
E) 15

What is proton-motive force?
A) the force required to remove an electron from hydrogen
B) the force exerted on a proton by a transmembrane proton concentration gradient
C) the force that moves hydrogen into the intermembrane space
D) the force that moves hydrogen into the mitochondrion
E) the force that moves hydrogen to NAD⁺

In liver cells, the inner mitochondrial membranes are about five times the area of the outer mitochondrial membranes. What purpose must this serve?
A) It allows for an increased rate of glycolysis.
B) It allows for an increased rate of the citric acid cycle.
C) It increases the surface for oxidative phosphorylation.
D) It increases the surface for substrate-level phosphorylation.
E) It allows the liver cell to have fewer mitochondria.

Brown fat cells produce a protein called thermogenin in their mitochondrial inner membrane. Thermogenin is a channel for facilitated transport of protons across the membrane. What will occur in the brown fat cells when they produce thermogenin?
A) ATP synthesis and heat generation will both increase.
B) ATP synthesis will increase, and heat generation will decrease.
C) ATP synthesis will decrease, and heat generation will increase.
D) ATP synthesis and heat generation will both decrease.
E) ATP synthesis and heat generation will stay the same.

In a mitochondrion, if the matrix ATP concentration is high, and the intermembrane space proton concentration is too low to generate sufficient proton-motive force, then
A) ATP synthase will increase the rate of ATP synthesis.
B) ATP synthase will stop working.
C) ATP synthase will hydrolyze ATP and pump protons into the intermembrane space.
D) ATP synthase will hydrolyze ATP and pump protons into the matrix.

Which catabolic processes may have been used by cells on ancient Earth before free oxygen became available?
A) glycolysis and fermentation only
B) glycolysis and the citric acid cycle only
C) glycolysis, pyruvate oxidation, and the citric acid cycle
D) oxidative phosphorylation only
E) glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation, using an electron acceptor other than oxygen

Which of the following normally occurs regardless of whether or not oxygen (O₂) is present?
A) glycolysis
B) fermentation
C) oxidation of pyruvate to acetyl CoA
D) citric acid cycle
E) oxidative phosphorylation (chemiosmosis)

Which of the following occurs in the cytosol of a eukaryotic cell?
A) glycolysis and fermentation
B) fermentation and chemiosmosis
C) oxidation of pyruvate to acetyl CoA
D) citric acid cycle
E) oxidative phosphorylation

Which metabolic pathway is common to both cellular respiration and fermentation?
A) the oxidation of pyruvate to acetyl CoA
B) the citric acid cycle
C) oxidative phosphorylation
D) glycolysis
E) chemiosmosis

The ATP made during fermentation is generated by which of the following?
A) the electron transport chain
B) substrate-level phosphorylation
C) chemiosmosis
D) oxidative phosphorylation
E) aerobic respiration

In the absence of oxygen, yeast cells can obtain energy by fermentation, resulting in the production of
A) ATP, CO₂, and ethanol (ethyl alcohol).
B) ATP, CO₂, and lactate.
C) ATP, NADH, and pyruvate.
D) ATP, pyruvate, and oxygen.
E) ATP, pyruvate, and acetyl CoA.

In alcohol fermentation, NAD⁺ is regenerated from NADH by
A) reduction of acetaldehyde to ethanol (ethyl alcohol).
B) oxidation of pyruvate to acetyl CoA.
C) reduction of pyruvate to form lactate.
D) oxidation of ethanol to acetyl CoA.
E) reduction of ethanol to pyruvate.

One function of both alcohol fermentation and lactic acid fermentation is to
A) reduce NAD⁺ to NADH.
B) reduce FAD⁺ to FADH₂.
C) oxidize NADH to NAD⁺.
D) reduce FADH₂ to FAD⁺.
E) do none of the above.

An organism is discovered that thrives both in the presence and absence of oxygen in the air. Curiously, the consumption of sugar increases as oxygen is removed from the organism's environment, even though the organism does not gain much weight. This organism
A) must use a molecule other than oxygen to accept electrons from the electron transport chain.
B) is a normal eukaryotic organism.
C) is photosynthetic.
D) is an anaerobic organism.
E) is a facultative anaerobe.

Which statement best supports the hypothesis that glycolysis is an ancient metabolic pathway that originated before the last universal common ancestor of life on Earth?
A) Glycolysis is widespread and is found in the domains Bacteria, Archaea, and Eukarya.
B) Glycolysis neither uses nor needs O₂.
C) Glycolysis is found in all eukaryotic cells.
D) The enzymes of glycolysis are found in the cytosol rather than in a membrane-enclosed organelle.
E) Ancient prokaryotic cells, the most primitive of cells, made extensive use of glycolysis long before oxygen was present in Earth's atmosphere.

Why is glycolysis considered to be one of the first metabolic pathways to have evolved?
A) It produces much less ATP than does oxidative phosphorylation.
B) It does not involve organelles or specialized structures, does not require oxygen, and is present in most organisms.
C) It is found in prokaryotic cells but not in eukaryotic cells.
D) It relies on chemiosmosis, which is a metabolic mechanism present only in the first cells' prokaryotic cells.
E) It requires the presence of membrane-enclosed cell organelles found only in eukaryotic cells.

When an individual is exercising heavily and when the muscle becomes oxygen-deprived, muscle cells convert pyruvate to lactate. What happens to the lactate in skeletal muscle cells?
A) It is converted to NAD⁺.
B) It produces CO₂ and water.
C) It is taken to the liver and converted back to pyruvate.
D) It reduces FADH₂ to FAD⁺.
E) It is converted to alcohol

When skeletal muscle cells are oxygen-deprived, the heart still pumps. What must the heart muscle cells be able to do?
A) derive sufficient energy from fermentation
B) continue aerobic metabolism when skeletal muscle cannot
C) transform lactate to pyruvate again
D) remove lactate from the blood
E) remove oxygen from lactate

When skeletal muscle cells undergo anaerobic respiration, they become fatigued and painful. This is now known to be caused by
A) buildup of pyruvate.
B) buildup of lactate.
C) increase in sodium ions.
D) increase in potassium ions.
E) increase in ethanol.

A mutation in yeast makes it unable to convert pyruvate to ethanol. How will this mutation affect these yeast cells?
A) The mutant yeast will be unable to grow anaerobically.
B) The mutant yeast will grow anaerobically only when given glucose.
C) The mutant yeast will be unable to metabolize glucose.
D) The mutant yeast will die because they cannot regenerate NAD⁺ from NAD.
E) The mutant yeast will metabolize only fatty acids.

You have a friend who lost 7 kg (about 15 pounds) of fat on a regimen of strict diet and exercise. How did the fat leave her body?
A) It was released as CO₂ and H₂O.
B) It was converted to heat and then released.
C) It was converted to ATP, which weighs much less than fat.
D) It was broken down to amino acids and eliminated from the body.
E) It was converted to urine and eliminated from the body.

Phosphofructokinase is an important control enzyme in the regulation of cellular respiration. Which of the following statements correctly describes phosphofructokinase activity?
A) It is inhibited by AMP.
B) It is activated by ATP.
C) It is activated by citrate, an intermediate of the citric acid cycle.
D) It catalyzes the conversion of fructose 1,6-bisphosphate to fructose 6-phosphate, an early step of glycolysis.
E) It is an allosteric enzyme.

Phosphofructokinase is an allosteric enzyme that catalyzes the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate, an early step of glycolysis. In the presence of oxygen, an increase in the amount of ATP in a cell would be expected to
A) inhibit the enzyme and thus slow the rates of glycolysis and the citric acid cycle.
B) activate the enzyme and thus slow the rates of glycolysis and the citric acid cycle.
C) inhibit the enzyme and thus increase the rates of glycolysis and the citric acid cycle.
D) activate the enzyme and increase the rates of glycolysis and the citric acid cycle.
E) inhibit the enzyme and thus increase the rate of glycolysis and the concentration of citrate.

Even though plants carry on photosynthesis, plant cells still use their mitochondria for oxidation of pyruvate. When and where will this occur?
A) in photosynthetic cells in the light, while photosynthesis occurs concurrently
B) in nonphotosynthesizing cells only
C) in cells that are storing glucose only
D) in all cells all the time
E) in photosynthesizing cells in the light and in other tissues in the dark

In vertebrate animals, brown fat tissue's color is due to abundant blood vessels and capillaries. White fat tissue, on the other hand, is specialized for fat storage and contains relatively few blood vessels or capillaries. Brown fat cells have a specialized protein that dissipates the proton-motive force across the mitochondrial membranes. Which of the following might be the function of the brown fat tissue?
A) to increase the rate of oxidative phosphorylation from its few mitochondria
B) to allow the animals to regulate their metabolic rate when it is especially hot
C) to increase the production of ATP
D) to allow other membranes of the cell to perform mitochondrial functions
E) to regulate temperature by converting most of the energy from NADH oxidation to heat

What is the purpose of beta oxidation in respiration?
A) oxidation of glucose
B) oxidation of pyruvate
C) feedback regulation
D) control of ATP accumulation
E) breakdown of fatty acids

Where do the catabolic products of fatty acid breakdown enter into the citric acid cycle?
A) pyruvate
B) malate or fumarate
C) acetyl CoA
D) α-ketoglutarate
E) succinyl CoA

What carbon sources can yeast cells metabolize to make ATP from ADP under anaerobic conditions?
A) glucose
B) ethanol
C) pyruvate
D) lactic acid
E) either ethanol or lactic acid

High levels of citric acid inhibit the enzyme phosphofructokinase, a key enzyme in glycolysis. Citric acid binds to the enzyme at a different location from the active site. This is an example of
A) competitive inhibition.
B) allosteric regulation.
C) the specificity of enzymes for their substrates.
D) an enzyme requiring a cofactor.
E) positive feedback regulation.

During intense exercise, as skeletal muscle cells go into anaerobiosis, the human body will increase its catabolism of
A) fats only.
B) carbohydrates only.
C) proteins only.
D) fats, carbohydrates, and proteins.
E) fats and proteins only.

Yeast cells that have defective mitochondria incapable of respiration will be able to grow by catabolizing which of the following carbon sources for energy?
A) glucose
B) proteins
C) fatty acids
D) glucose, proteins, and fatty acids
E) Such yeast cells will not be capable of catabolizing any food molecules, and will therefore die.

Which step in Figure 9.1 shows a split of one molecule into two smaller molecules?
A) A
B) B
C) C
D) D
E) E

In which step in Figure 9.1 is an inorganic phosphate added to the reactant?
A) A
B) B
C) C
D) D
E) E

Which step in Figure 9.1 is a redox reaction?
A) A
B) B
C) C
D) D
E) E

Which portion of the pathway in Figure 9.1 involves an endergonic reaction?
A) A
B) B
C) C
D) D
E) E

Which portion of the pathway in Figure 9.1 contains a phosphorylation reaction in which ATP is the phosphate source?
A) A
B) B
C) C
D) D
E) E

Starting with one molecule of isocitrate and ending with fumarate, how many ATP molecules can be made through substrate-level phosphorylation (see Figure 9.2)?
A) 1
B) 2
C) 11
D) 12
E) 24

Carbon skeletons for amino acid biosynthesis are supplied by intermediates of the citric acid cycle. Which intermediate would supply the carbon skeleton for synthesis of a five-carbon amino acid (see Figure 9.2)?
A) succinate
B) malate
C) citrate
D) α-ketoglutarate
E) isocitrate

For each mole of glucose (C₆H₁₂O₆) oxidized by cellular respiration, how many moles of CO₂ are released in the citric acid cycle (see Figure 9.2)?
A) 2
B) 4
C) 6
D) 12
E) 3

If pyruvate oxidation is blocked, what will happen to the levels of oxaloacetate and citric acid in the citric acid cycle shown in Figure 9.2?
A) There will be no change in the levels of oxaloacetate and citric acid.
B) Oxaloacetate will decrease and citric acid will accumulate.
C) Oxaloacetate will accumulate and citric acid will decrease.
D) Both oxaloacetate and citric acid will decrease.
E) Both oxaloacetate and citric acid will accumulate.

Answer: C

Starting with citrate, which of the following combinations of products would result from three acetyl CoA molecules entering the citric acid cycle (see Figure 9.2)?
A) 1 ATP, 2 CO₂, 3 NADH, and 1 FADH₂
B) 2 ATP, 2 CO₂, 3 NADH, and 3 FADH₂
C) 3 ATP, 3 CO₂, 3 NADH, and 3 FADH₂
D) 3 ATP, 6 CO₂, 9 NADH, and 3 FADH₂
E) 38 ATP, 6 CO₂, 3 NADH, and 12 FADH₂

For each molecule of glucose that is metabolized by glycolysis and the citric acid cycle (see Figure 9.2), what is the total number of NADH + FADH₂ molecules produced?
A) 4
B) 5
C) 6
D) 10
E) 12

Figure 9.3 shows the electron transport chain. Which of the following is the combination of substances that is initially added to the chain?
A) oxygen, carbon dioxide, and water
B) NAD⁺, FAD, and electrons
C) NADH, FADH₂, and protons
D) NADH, FADH₂, and O₂
E) oxygen and protons

Which of the following most accurately describes what is happening along the electron transport chain in Figure 9.3?
A) Chemiosmosis is coupled with electron transfer.
B) Each electron carrier alternates between being reduced and being oxidized.
C) ATP is generated at each step.
D) Energy of the electrons increases at each step.
E) Molecules in the chain give up some of their potential energy.

Which of the protein complexes labeled with Roman numerals in Figure 9.3 will transfer electrons to O₂?
A) complex I
B) complex II
C) complex III
D) complex IV
E) All of the complexes can transfer electrons to O₂.

What happens at the end of the chain in Figure 9.3?
A) 2 electrons combine with a proton and a molecule of NAD⁺.
B) 2 electrons combine with a molecule of oxygen and two hydrogen atoms.
C) 4 electrons combine with a molecule of oxygen and 4 protons.
D) 4 electrons combine with four hydrogen and two oxygen atoms.
E) 1 electron combines with a molecule of oxygen and a hydrogen atom.

In the presence of oxygen, the three-carbon compound pyruvate can be catabolized in the citric acid cycle. First, however, the pyruvate (1) loses a carbon, which is given off as a molecule of CO₂, (2) is oxidized to form a two-carbon compound called acetate, and (3) is bonded to coenzyme A.

These three steps result in the formation of
A) acetyl CoA, O₂, and ATP.
B) acetyl CoA, FADH₂, and CO₂.
C) acetyl CoA, FAD, H₂, and CO₂.
D) acetyl CoA, NADH, H⁺, and CO₂.
E) acetyl CoA, NAD⁺, ATP, and CO₂.

In the presence of oxygen, the three-carbon compound pyruvate can be catabolized in the citric acid cycle. First, however, the pyruvate (1) loses a carbon, which is given off as a molecule of CO₂, (2) is oxidized to form a two-carbon compound called acetate, and (3) is bonded to coenzyme A.

Why is coenzyme A, a sulfur-containing molecule derived from a B vitamin, added?
A) because sulfur is needed for the molecule to enter the mitochondrion
B) in order to utilize this portion of a B vitamin which would otherwise be a waste product from another pathway
C) to provide a relatively unstable molecule whose acetyl portion can be readily transferred to a compound in the citric acid cycle
D) because it drives the reaction that regenerates NAD⁺
E) in order to remove one molecule of CO₂

Exposing inner mitochondrial membranes to ultrasonic vibrations will disrupt the membranes. However, the fragments will reseal "inside out." These little vesicles that result can still transfer electrons from NADH to oxygen and synthesize ATP. If the membranes are agitated further, however, the ability to synthesize ATP is lost.

After the first disruption, when electron transfer and ATP synthesis still occur, what must be present?
A) all of the electron transport proteins as well as ATP synthase
B) all of the electron transport system and the ability to add CoA to acetyl groups
C) the ATP synthase system
D) the electron transport system
E) plasma membranes like those bacteria use for respiration

Exposing inner mitochondrial membranes to ultrasonic vibrations will disrupt the membranes. However, the fragments will reseal "inside out." These little vesicles that result can still transfer electrons from NADH to oxygen and synthesize ATP. If the membranes are agitated further, however, the ability to synthesize ATP is lost.

After the further agitation of the membrane vesicles, what must be lost from the membrane?
A) the ability of NADH to transfer electrons to the first acceptor in the electron transport chain
B) the prosthetic groups like heme from the transport system
C) cytochromes
D) ATP synthase, in whole or in part
E) the contact required between inner and outer membrane surfaces

Exposing inner mitochondrial membranes to ultrasonic vibrations will disrupt the membranes. However, the fragments will reseal "inside out." These little vesicles that result can still transfer electrons from NADH to oxygen and synthesize ATP. If the membranes are agitated further, however, the ability to synthesize ATP is lost.

These inside-out membrane vesicles
A) will become acidic inside the vesicles when NADH is added.
B) will become alkaline inside the vesicles when NADH is added.
C) will make ATP from ADP and i if transferred to a pH 4 buffered solution after incubation in a pH 7 buffered solution.
D) will hydrolyze ATP to pump protons out of the interior of the vesicle to the exterior.
E) will reverse electron flow to generate NADH from NAD⁺ in the absence of oxygen.

The immediate energy source that drives ATP synthesis by ATP synthase during oxidative phosphorylation is the
A) oxidation of glucose and other organic compounds.
B) flow of electrons down the electron transport chain.
C) affinity of oxygen for electrons.
D) H⁺ concentration across the membrane holding ATP synthase.
E) transfer of phosphate to ADP.

Which metabolic pathway is common to both fermentation and cellular respiration of a glucose molecule?
A) the citric acid cycle
B) the electron transport chain
C) glycolysis
D) synthesis of acetyl CoA from pyruvate
E) reduction of pyruvate to lactate

In mitochondria, exergonic redox reactions
A) are the source of energy driving prokaryotic ATP synthesis.
B) are directly coupled to substrate-level phosphorylation.
C) provide the energy that establishes the proton gradient.
D) reduce carbon atoms to carbon dioxide.
E) are coupled via phosphorylated intermediates to endergonic processes.

The final electron acceptor of the electron transport chain that functions in aerobic oxidative phosphorylation is
A) oxygen.
B) water.
C) NAD⁺.
D) pyruvate.
E) ADP.

What is the oxidizing agent in the following reaction?
Pyruvate + NADH + H⁺ → Lactate + NAD⁺
A) oxygen
B) NADH
C) NAD⁺
D) lactate
E) pyruvate

When electrons flow along the electron transport chains of mitochondria, which of the following changes occurs?
A) The pH of the matrix increases.
B) ATP synthase pumps protons by active transport.
C) The electrons gain free energy.
D) The cytochromes phosphorylate ADP to form ATP.
E) NAD⁺ is oxidized.

Most CO₂ from catabolism is released during
A) glycolysis.
B) the citric acid cycle.
C) lactate fermentation.
D) electron transport.
E) oxidative phosphorylation.

If photosynthesizing green algae are provided with CO₂ synthesized with heavy oxygen (¹⁸O), later analysis will show that all but one of the following compounds produced by the algae contain the ¹⁸O label. That one is
A) 3-phosphoglycerate.
B) glyceraldehyde 3-phosphate (G3P).
C) glucose.
D) ribulose bisphosphate (RuBP).
E) O₂.

Which of the following are products of the light reactions of photosynthesis that are utilized in the Calvin cycle?
A) CO₂ and glucose
B) H₂O and O₂
C) ADP, Pi, and NADP⁺
D) electrons and H⁺
E) ATP and NADPH

Photosynthesis is not responsible for
A) oxygen in the atmosphere.
B) the ozone layer.
C) most of the organic carbon on Earth's surface.
D) atmospheric CO₂.
E) fossil fuels.

Where does the Calvin cycle take place?
A) stroma of the chloroplast
B) thylakoid membrane
C) cytoplasm surrounding the chloroplast
D) interior of the thylakoid (thylakoid space)
E) outer membrane of the chloroplast

In any ecosystem, terrestrial or aquatic, what group(s) is (are) always necessary?
A) autotrophs and heterotrophs
B) producers and primary consumers
C) photosynthesizers
D) autotrophs
E) green plants

In autotrophic bacteria, where are the enzymes located that can carry on carbon fixation (reduction of carbon dioxide to carbohydrate)?
A) in chloroplast membranes
B) in chloroplast stroma
C) in the cytosol
D) in the nucleoid
E) in the infolded plasma membrane

When oxygen is released as a result of photosynthesis, it is a direct by-product of
A) reducing NADP⁺.
B) splitting water molecules.
C) chemiosmosis.
D) the electron transfer system of photosystem I.
E) the electron transfer system of photosystem II.

A plant has a unique photosynthetic pigment. The leaves of this plant appear to be reddish yellow. What wavelengths of visible light are being absorbed by this pigment?
A) red and yellow
B) blue and violet
C) green and yellow
D) blue, green, and red
E) green, blue, and yellow

Halobacterium has a photosynthetic membrane that is colored purple. Its photosynthetic action spectrum is exactly complementary (opposite to) the action spectrum for green plants. What wavelengths of light do the Halobacterium photosynthetic pigments absorb?
A) red and yellow
B) blue, green, and red
C) green and yellow
D) red and green
E) blue and red

In the thylakoid membranes, what is the main role of the antenna pigment molecules?
A) split water and release oxygen to the reaction-center chlorophyll
B) harvest photons and transfer light energy to the reaction-center chlorophyll
C) synthesize ATP from ADP and Pi
D) transfer electrons to ferredoxin and then NADPH
E) concentrate photons within the stroma

Which of the events listed below occurs in the light reactions of photosynthesis?
A) NADP is produced.
B) NADPH is reduced to NADP⁺.
C) Carbon dioxide is incorporated into PGA.
D) ATP is phosphorylated to yield ADP.
E) Light is absorbed and funneled to reaction-center chlorophyll a.

Which statement describes the functioning of photosystem II?
A) Light energy excites electrons in the thylakoid membrane electron transport chain.
B) Photons are passed along to a reaction-center chlorophyll.
C) The P680 chlorophyll donates a pair of protons to NADP⁺, which is thus converted to NADPH.
D) The electron vacancies in P680⁺ are filled by electrons derived from water.
E) The splitting of water yields molecular carbon dioxide as a by-product.

Which of the following are directly associated with photosystem I?
A) harvesting of light energy by ATP
B) receiving electrons from the thylakoid membrane electron transport chain
C) generation of molecular oxygen
D) extraction of hydrogen electrons from the splitting of water
E) passing electrons to the thylakoid membrane electron transport chain

Some photosynthetic organisms contain chloroplasts that lack photosystem II, yet are able to survive. The best way to detect the lack of photosystem II in these organisms would be
A) to determine if they have thylakoids in the chloroplasts.
B) to test for liberation of O₂ in the light.
C) to test for CO₂ fixation in the dark.
D) to do experiments to generate an action spectrum.
E) to test for production of either sucrose or starch.

What are the products of linear photophosphorylation?
A) heat and fluorescence
B) ATP and P700
C) ATP and NADPH
D) ADP and NADP
E) P700 and P680

As a research scientist, you measure the amount of ATP and NADPH consumed by the Calvin cycle in 1 hour. You find 30,000 molecules of ATP consumed, but only 20,000 molecules of NADPH. Where did the extra ATP molecules come from?
A) photosystem II
B) photosystem I
C) cyclic electron flow
D) linear electron flow
E) chlorophyll

Assume a thylakoid is somehow punctured so that the interior of the thylakoid is no longer separated from the stroma. This damage will have the most direct effect on which of the following processes?
A) the splitting of water
B) the absorption of light energy by chlorophyll
C) the flow of electrons from photosystem II to photosystem I
D) the synthesis of ATP
E) the reduction of NADP⁺

What does the chemiosmotic process in chloroplasts involve?
A) establishment of a proton gradient across the thylakoid membrane
B) diffusion of electrons through the thylakoid membrane
C) reduction of water to produce ATP energy
D) movement of water by osmosis into the thylakoid space from the stroma
E) formation of glucose, using carbon dioxide, NADPH, and ATP

Suppose the interior of the thylakoids of isolated chloroplasts were made acidic and then transferred in the dark to a pH 8 solution. What would be likely to happen?
A) The isolated chloroplasts will make ATP.
B) The Calvin cycle will be activated.
C) Cyclic photophosphorylation will occur.
D) The isolated chloroplasts will generate oxygen gas.
E) The isolated chloroplasts will reduce NADP⁺ to NADPH.

In a plant cell, where are the ATP synthase complexes located?
A) thylakoid membrane only
B) plasma membrane only
C) inner mitochondrial membrane only
D) thylakoid membrane and inner mitochondrial membrane
E) thylakoid membrane and plasma membrane

In mitochondria, chemiosmosis translocates protons from the matrix into the intermembrane space, whereas in chloroplasts, chemiosmosis translocates protons from
A) the stroma to the photosystem II.
B) the matrix to the stroma.
C) the stroma to the thylakoid space.
D) the intermembrane space to the matrix.
E) the thylakoid space to the stroma.

Which of the following statements best describes the relationship between photosynthesis and respiration?
A) Respiration runs the biochemical pathways of photosynthesis in reverse.
B) Photosynthesis stores energy in complex organic molecules, whereas respiration releases it.
C) Photosynthesis occurs only in plants and respiration occurs only in animals.
D) ATP molecules are produced in photosynthesis and used up in respiration.
E) Respiration is anabolic and photosynthesis is catabolic.

Where are the molecules of the electron transport chain found in plant cells?
A) thylakoid membranes of chloroplasts
B) stroma of chloroplasts
C) outer membrane of mitochondria
D) matrix of mitochondria
E) cytoplasm

In photosynthetic cells, synthesis of ATP by the chemiosmotic mechanism occurs during
A) photosynthesis only.
B) respiration only.
C) both photosynthesis and respiration.
D) neither photosynthesis nor respiration.
E) photorespiration only.

Reduction of oxygen to form water occurs during
A) photosynthesis only.
B) respiration only.
C) both photosynthesis and respiration.
D) neither photosynthesis nor respiration.
E) photorespiration only.

Reduction of NADP⁺ occurs during
A) photosynthesis.
B) respiration.
C) both photosynthesis and respiration.
D) neither photosynthesis nor respiration.
E) photorespiration.

The splitting of carbon dioxide to form oxygen gas and carbon compounds occurs during
A) photosynthesis.
B) respiration.
C) both photosynthesis and respiration.
D) neither photosynthesis nor respiration.
E) photorespiration.

Generation of proton gradients across membranes occurs during
A) photosynthesis.
B) respiration.
C) both photosynthesis and respiration.
D) neither photosynthesis nor respiration.
E) photorespiration.

What is the relationship between wavelength of light and the quantity of energy per photon?
A) They have a direct, linear relationship.
B) They are inversely related.
C) They are logarithmically related.
D) They are separate phenomena.
E) They are only related in certain parts of the spectrum.

P680⁺ is said to be the strongest biological oxidizing agent. Why?
A) It is the receptor for the most excited electron in either photosystem.
B) It is the molecule that transfers electrons to plastoquinone (Pq) of the electron transfer system.
C) It transfers its electrons to reduce NADP⁺ to NADPH.
D) This molecule has a stronger attraction for electrons than oxygen, to obtain electrons from water.
E) It has a positive charge.

Some photosynthetic bacteria (e.g., purple sulfur bacteria) have only photosystem I, whereas others (e.g., cyanobacteria) have both photosystem I and photosystem II. Which of the following might this observation imply?
A) Photosystem II was selected against in some species.
B) Photosynthesis with only photosystem I is more ancestral.
C) Photosystem II may have evolved to be more photoprotective.
D) Linear electron flow is more primitive than cyclic flow of electrons.
E) Cyclic flow is more necessary than linear electron flow.

electron flow may be photoprotective (protective to light-induced damage). Which of the following experiments could provide information on this phenomenon?
A) use mutated organisms that can grow but that cannot carry out cyclic flow of electrons and compare their abilities to photosynthesize in different light intensities against those of wild-type organisms
B) use plants that can carry out both linear and cyclic electron flow, or only one or another of these processes, and compare their light absorbance at different wavelengths and different light intensities
C) use bacteria that have only cyclic flow and look for their frequency of mutation damage at different light intensities
D) use bacteria with only cyclic flow and measure the number and types of photosynthetic pigments they have in their membranes
E) use plants with only photosystem I operative and measure how much damage occurs at different wavelengths

Carotenoids are often found in foods that are considered to have antioxidant properties in human nutrition. What related function do they have in plants?
A) They serve as accessory pigments to increase light absorption.
B) They protect against oxidative damage from excessive light energy.
C) They shield the sensitive chromosomes of the plant from harmful ultraviolet radiation.
D) They reflect orange light and enhance red light absorption by chlorophyll.
E) They take up and remove toxins from the groundwater.

In thylakoids, protons travel through ATP synthase from the thylakoid space to the stroma. Therefore, the catalytic "knobs" of ATP synthase would be located
A) on the side facing the thylakoid space.
B) on the ATP molecules themselves.
C) on the pigment molecules of photosystem I and photosystem II.
D) on the stromal side of the membrane.
E) built into the center of the thylakoid stack (granum).

In metabolic processes of cell respiration and photosynthesis, prosthetic groups such as heme and iron-sulfur complexes are encountered in components of the electron transport chain. What do they do?
A) donate electrons
B) act as reducing agents
C) act as oxidizing agents
D) transport protons within the mitochondria and chloroplasts
E) both oxidize and reduce during electron transport

In a cyanobacterium, the reactions that produce NADPH occur in
A) the light reactions alone.
B) the Calvin cycle alone.
C) both the light reactions and the Calvin cycle.
D) neither the light reactions nor the Calvin cycle.
E) the chloroplast, but is not part of photosynthesis.

The reactions that produce molecular oxygen (O₂) take place in
A) the light reactions alone.
B) the Calvin cycle alone.
C) both the light reactions and the Calvin cycle.
D) neither the light reactions nor the Calvin cycle.
E) the chloroplast, but are not part of photosynthesis

The accumulation of free oxygen in Earth's atmosphere began
A) with the origin of life and respiratory metabolism.
B) with the origin of photosynthetic bacteria that had photosystem I.
C) with the origin of cyanobacteria that had both photosystem I and photosystem II.
D) with the origin of chloroplasts in photosynthetic eukaryotic algae.
E) with the origin of land plants.

A flask containing photosynthetic green algae and a control flask containing water with no algae are both placed under a bank of lights, which are set to cycle between 12 hours of light and 12 hours of dark. The dissolved oxygen concentrations in both flasks are monitored. Predict what the relative dissolved oxygen concentrations will be in the flask with algae compared to the control flask.
A) The dissolved oxygen in the flask with algae will always be higher.
B) The dissolved oxygen in the flask with algae will always be lower.
C) The dissolved oxygen in the flask with algae will be higher in the light, but the same in the dark.
D) The dissolved oxygen in the flask with algae will be higher in the light, but lower in the dark.
E) The dissolved oxygen in the flask with algae will not be different from the control flask at any time.

Where do the enzymatic reactions of the Calvin cycle take place?
A) stroma of the chloroplast
B) thylakoid membranes
C) matrix of the mitochondria
D) cytosol around the chloroplast
E) thylakoid space

What is the primary function of the Calvin cycle?
A) use ATP to release carbon dioxide
B) use NADPH to release carbon dioxide
C) split water and release oxygen
D) transport RuBP out of the chloroplast
E) synthesize simple sugars from carbon dioxide

In C₃ photosynthesis, the reactions that require ATP take place in
A) the light reactions alone.
B) the Calvin cycle alone.
C) both the light reactions and the Calvin cycle.
D) neither the light reactions nor the Calvin cycle.
E) the chloroplast, but is not part of photosynthesis.

In a plant leaf, the reactions that produce NADH occur in
A) the light reactions alone.
B) the Calvin cycle alone.
C) both the light reactions and the Calvin cycle.
D) neither the light reactions nor the Calvin cycle.
E) the chloroplast, but is not part of photosynthesis.

The NADPH required for the Calvin cycle comes from
A) reactions initiated in photosystem I.
B) reactions initiated in photosystem II.
C) the citric acid cycle.
D) glycolysis.
E) oxidative phosphorylation.

Reactions that require CO₂ take place in
A) the light reactions alone.
B) the Calvin cycle alone.
C) both the light reactions and the Calvin cycle.
D) neither the light reactions nor the Calvin cycle.
E) the chloroplast, but is not part of photosynthesis.

Which of the following statements best represents the relationships between the light reactions and the Calvin cycle?
A) The light reactions provide ATP and NADPH to the Calvin cycle, and the cycle returns ADP, Pi, and NADP⁺ to the light reactions.
B) The light reactions provide ATP and NADPH to the carbon fixation step of the Calvin cycle, and the cycle provides water and electrons to the light reactions.
C) The light reactions supply the Calvin cycle with CO₂ to produce sugars, and the Calvin cycle supplies the light reactions with sugars to produce ATP.
D) The light reactions provide the Calvin cycle with oxygen for electron flow, and the Calvin cycle provides the light reactions with water to split.
E) There is no relationship between the light reactions and the Calvin cycle.

Three "turns" of the Calvin cycle generate a "surplus" molecule of glyceraldehyde 3-phosphate (G3P). Which of the following is a consequence of this?
A) Formation of a molecule of glucose would require nine "turns."
B) G3P more readily forms sucrose and other disaccharides than it does monosaccharides.
C) Some plants would not taste sweet to us.
D) The formation of sucrose and starch in plants involves assembling G3P molecules, with or without further rearrangements.
E) Plants accumulate and store G3P.

In the process of carbon fixation, RuBP attaches a CO₂ to produce a six-carbon molecule, which is then split to produce two molecules of 3-phosphoglycerate. After phosphorylation and reduction produces glyceraldehyde 3-phosphate (G3P), what more needs to happen to complete the Calvin cycle?
A) addition of a pair of electrons from NADPH
B) inactivation of RuBP carboxylase enzyme
C) regeneration of ATP from ADP
D) regeneration of RuBP
E) regeneration of NADP⁺

The pH of the inner thylakoid space has been measured, as have the pH of the stroma and of the cytosol of a particular plant cell. Which, if any, relationship would you expect to find?
A) The pH within the thylakoid is less than that of the stroma.
B) The pH of the stroma is lower than that of the other two measurements.
C) The pH of the stroma is higher than that of the thylakoid space but lower than that of the cytosol.
D) The pH of the thylakoid space is higher than that anywhere else in the cell.
E) There is no consistent relationship.

The phylogenetic distribution of the enzyme rubisco is limited to
A) C₃ plants only.
B) C₃ and C₄ plants.
C) all photosynthetic eukaryotes.
D) all known photoautotrophs, both bacterial and eukaryotic.
E) all living cells.

Photorespiration occurs when rubisco reacts RuBP with
A) CO₂.
B) O₂.
C) glyceraldehyde 3-phosphate.
D) 3-phosphoglycerate.
E) NADPH.

In an experiment studying photosynthesis performed during the day, you provide a plant with radioactive carbon (¹⁴C) dioxide as a metabolic tracer. The ¹⁴C is incorporated first into oxaloacetate. The plant is best characterized as a
A) C₄ plant.
B) C₃ plant.
C) CAM plant.
D) heterotroph.
E) chemoautotroph.

Why are C₄ plants able to photosynthesize with no apparent photorespiration?
A) They do not participate in the Calvin cycle.
B) They use PEP carboxylase to initially fix CO₂.
C) They are adapted to cold, wet climates.
D) They conserve water more efficiently.
E) They exclude oxygen from their tissues.

CAM plants keep stomata closed in daytime, thus reducing loss of water. They can do this because they
A) fix CO₂ into organic acids during the night.
B) fix CO₂ into sugars in the bundle-sheath cells.
C) fix CO₂ into pyruvate in the mesophyll cells.
D) use the enzyme phosphofructokinase, which outcompetes rubisco for CO₂.
E) use photosystem I and photosystem II at night.

Photorespiration lowers the efficiency of photosynthesis by
A) carbon dioxide molecules.
B) 3-phosphoglycerate molecules.
C) ATP molecules.
D) ribulose bisphosphate molecules.
E) RuBP carboxylase molecules.

The alternative pathways of photosynthesis using the C₄ or CAM systems are said to be compromises. Why?
A) Each one minimizes both water loss and rate of photosynthesis.
B) C₄ compromises on water loss and CAM compromises on photorespiration.
C) Both minimize photorespiration but expend more ATP during carbon fixation.
D) CAM plants allow more water loss, while C₄ plants allow less CO₂ into the plant.
E) C₄ plants allow less water loss but CAM plants allow more water loss.

If plant gene alterations cause the plants to be deficient in photorespiration, what would most probably occur?
A) Photosynthetic efficiency would be reduced at low light intensities.
B) Cells would carry on the Calvin cycle at a much slower rate.
C) Less ATP would be generated.
D) There would be more light-induced damage to the cells.
E) Less oxygen would be produced.

Compared to C₃ plants, C₄ plants
A) can continue to fix CO₂ even at relatively low CO2 concentrations and high oxygen concentrations.
B) have higher rates of photorespiration.
C) do not use rubisco for carbon fixation.
D) grow better under cool, moist conditions.
E) make a four-carbon compound, oxaloacetate, which is then delivered to the citric acid cycle in mitochondria.

If atmospheric CO₂ concentrations increase twofold or more, how will plants be affected, disregarding any changes in climate?

A) All plants will experience increased rates of photosynthesis.

B) C₃ plants will have faster growth; C₄ plants will be minimally affected.

C) C₄ plants will have faster growth; C₃ plants will be minimally affected.

D) C₃ plants will have faster growth; C₄ plants will have slower growth.

E) Plant growth will not be affected because atmospheric CO₂ concentrations are never limiting for plant growth.

Plants photosynthesize only in the light. Plants respire
A) in the dark only.
B) in the light only.
C) both in light and dark.
D) never–they get their ATP from photophosphorylation.
E) only when excessive light energy induces photorespiration.

Figure 10.1 shows the absorption spectrum for chlorophyll a and the action spectrum for photosynthesis. Why are they different?
A) Green and yellow wavelengths inhibit the absorption of red and blue wavelengths.
B) Bright sunlight destroys photosynthetic pigments.
C) Oxygen given off during photosynthesis interferes with the absorption of light.
D) Other pigments absorb light in addition to chlorophyll a.
E) Aerobic bacteria take up oxygen, which changes the measurement of the rate of photosynthesis.

What wavelength of light in the figure is most effective in driving photosynthesis?
A) 420 mm
B) 475 mm
C) 575 mm
D) 625 mm
E) 730 mm

If ATP used by this plant is labeled with radioactive phosphorus, which molecule or molecules of the Calvin cycle will be radioactively labeled first?
A) B only
B) B and C only
C) B, C, and D only
D) B and E only
E) B, C, D, and E

If the carbon atom of the incoming CO₂ molecule is labeled with a radioactive isotope of carbon, which organic molecules will be radioactively labeled after one cycle?
A) C only
B) B, C, D, and E
C) C, D, and E only
D) B and C only
E) B and D only

Which molecule(s) of the Calvin cycle is (are) also found in glycolysis?
A) B, C, E, and 3-phosphoglycerate
B) B, C, and E only
C) 3-phosphoglycerate only
D) B, C, D, and 3-phosphoglycerate only
E) E only

To identify the molecule that accepts CO₂, Calvin and Benson manipulated the carbon-fixation cycle by either cutting off CO₂ or cutting off light from cultures of photosynthetic algae. They then measured the concentrations of various metabolites immediately following the manipulation. How would these experiments help identify the CO₂ acceptor? Study Figure 10.2 to help you in determining the correct answer.
A) The CO₂ acceptor concentration would decrease when either the CO₂ or light are cut off.
B) The CO₂ acceptor concentration would increase when either the CO₂ or light are cut off.
C) The CO₂ acceptor concentration would increase when the CO₂ is cut off, but decrease when the light is cut off.
D) The CO₂ acceptor concentration would decrease when the CO₂ is cut off, but increase when the light is cut off.
E) The CO₂ acceptor concentration would stay the same regardless of the CO₂ or light.

Which of the following statements is true concerning Figure 10.3?
A) It represents cell processes involved in C₄ photosynthesis.
B) It represents the type of cell structures found in CAM plants.
C) It represents an adaptation that maximizes photorespiration.
D) It represents a C₃ photosynthetic system.
E) It represents a relationship between plant cells that photosynthesize and those that cannot.

Referring to Figure 10.3, oxygen would inhibit the CO₂ fixation reactions in
A) cell I only.
B) cell II only.
C) neither cell I nor cell II.
D) both cell I and cell II.
E) cell I during the night and cell II during the day.

A gardener is concerned that her greenhouse is getting too hot from too much light, and seeks to shade her plants with colored translucent plastic sheets. What color should she use to reduce overall light energy, but still maximize plant growth?
A) green
B) blue
C) yellow
D) orange
E) any color will work equally well

Theodor W. Engelmann illuminated a filament of algae with light that passed through a prism, thus exposing different segments of algae to different wavelengths of light. He added aerobic bacteria and then noted in which areas the bacteria congregated. He noted that the largest groups were found in the areas illuminated by the red and blue light.

What did Engelmann conclude about the congregation of bacteria in the red and blue areas?
A) Bacteria released excess carbon dioxide in these areas.
B) Bacteria congregated in these areas due to an increase in the temperature of the red and blue light.
C) Bacteria congregated in these areas because these areas had the most oxygen being released.
D) Bacteria are attracted to red and blue light and thus these wavelengths are more reactive than other wavelengths.
E) Bacteria congregated in these areas due to an increase in the temperature caused by an increase in photosynthesis.

Theodor W. Engelmann illuminated a filament of algae with light that passed through a prism, thus exposing different segments of algae to different wavelengths of light. He added aerobic bacteria and then noted in which areas the bacteria congregated. He noted that the largest groups were found in the areas illuminated by the red and blue light.

An outcome of this experiment was to help determine
A) the relationship between heterotrophic and autotrophic organisms.
B) the relationship between wavelengths of light and the rate of aerobic respiration.
C) the relationship between wavelengths of light and the amount of heat released.
D) the relationship between wavelengths of light and the rate of photosynthesis.
E) the relationship between the concentration of carbon dioxide and the rate of photosynthesis.

Theodor W. Engelmann illuminated a filament of algae with light that passed through a prism, thus exposing different segments of algae to different wavelengths of light. He added aerobic bacteria and then noted in which areas the bacteria congregated. He noted that the largest groups were found in the areas illuminated by the red and blue light.

If you ran the same experiment without passing light through a prism, what would you predict?
A) There would be no difference in results.
B) The bacteria would be relatively evenly distributed along the algal filaments.
C) The number of bacteria present would decrease due to an increase in the carbon dioxide concentration.
D) The number of bacteria present would increase due to an increase in the carbon dioxide concentration.
E) The number of bacteria would decrease due to a decrease in the temperature of the water.

A spaceship is designed to support animal life for a multiyear voyage to the outer planets of the solar system. Plants will be grown to provide oxygen and to recycle carbon dioxide.

Since the spaceship will be too far from the sun for photosynthesis, an artificial light source will be needed. What wavelengths of light should be used to maximize plant growth with a minimum of energy expenditure?
A) full-spectrum white light
B) green light
C) a mixture of blue and red light
D) yellow light
E) UV light

A spaceship is designed to support animal life for a multiyear voyage to the outer planets of the solar system. Plants will be grown to provide oxygen and to recycle carbon dioxide.

If the power fails and the lights go dark, what will happen to CO₂ levels?
A) CO₂ will rise as a result of both animal and plant respiration.
B) CO₂ will rise as a result of animal respiration only.
C) CO₂ will remain balanced because plants will continue to fix CO₂ in the dark.
D) CO₂ will fall because plants will increase CO₂ fixation.
E) CO₂ will fall because plants will cease to respire in the dark.

The light reactions of photosynthesis supply the Calvin cycle with
A) light energy.
B) CO₂ and ATP.
C) H₂O and NADPH.
D) ATP and NADPH.
E) sugar and O₂.

Which of the following sequences correctly represents the flow of electrons during photosynthesis?
A) NADPH → O₂ → CO₂
B) H₂O → NADPH → Calvin cycle
C) NADPH → chlorophyll → Calvin cycle
D) H₂O → photosystem I → photosystem II
E) NADPH → electron transport chain → O₂

How is photosynthesis similar in C₄ plants and CAM plants?
A) In both cases, only photosystem I is used.
B) Both types of plants make sugar without the Calvin cycle.
C) In both cases, rubisco is not used to fix carbon initially.
D) Both types of plants make most of their sugar in the dark.
E) In both cases, thylakoids are not involved in photosynthesis.

Which of the following statements is a correct distinction between autotrophs and heterotrophs?
A) Only heterotrophs require chemical compounds from the environment.
B) Cellular respiration is unique to heterotrophs.
C) Only heterotrophs have mitochondria.
D) Autotrophs, but not heterotrophs, can nourish themselves beginning with CO₂ and other nutrients that are inorganic.
E) Only heterotrophs require oxygen.

Which of the following does not occur during the Calvin cycle?
A) carbon fixation
B) oxidation of NADPH
C) release of oxygen
D) regeneration of the CO₂ acceptor
E) consumption of ATP

In mechanism, photophosphorylation is most similar to
A) substrate-level phosphorylation in glycolysis.
B) oxidative phosphorylation in cellular respiration.
C) the Calvin cycle.
D) carbon fixation.
E) reduction of NADP⁺.

Which process is most directly driven by light energy?
A) creation of a pH gradient by pumping protons across the thylakoid membrane
B) carbon fixation in the stroma
C) reduction of NADP⁺ molecules
D) removal of electrons from chlorophyll molecules
E) ATP synthesis