Functional anatomy of prokaryotic and eukaryotic cells
they lack a plasma membrane
Which of the following statement is incorrect regarding prokaryotic cells
it protects the cell in a hypertonic environment
Each of the following statements concerning the gram-positive cell wall is true except
water will move out of the cell
Which of the following statements best describes what happens when a bacterial cell is placed in a solution containing 5% NaCl
conjugation
A gram-positive bacterium suddenly acquires resistance to the antibiotic methicillin. this trait most likely occurred due to acquisition of new genetic information through
active transport
by which of the following mechanisms can a cell transport a substance from a lower to a higher concentration
contains cholesterol
which of the following is not a typical characteristic of most bacterial plasma membranes
fungi
which one of the following organisms has a cell wall
endospores allow a cell to survive environmental changes by producing a dormant period with no growth
which of the following statements is true
centrosomes - food storage
which of the following pairs is mismatched
mitochondrion
which of the following organelles most likely resembles a prokaryotic cell
B
Which of the drawing is a tetrad
A
which drawing in figure 4.1 posseses an axial filament
D
which drawing in figure in 4.1 is streptococci
osmotic lysis
antibiotics that target cell wall synthesis ultimately cause bacterial cell death as a result of
lysozyme
bacteria are commonly used organism for studies of genetic material in the research laboratory. The nucleic acids must first be isolated from the cells for these studies. which of the following would most likely be used to lyse gram-positive bacterial cells for nucleic acid isolation
they are sensitive to penicillin
which of the following statements about gram-negative cell walls is false
cilium
which of the following structures is not found in some prokayotic cells
binary fission
functions of the glycocalyx include all of the following except
capsule
which acts like an invisibility cloak and protects bacteria from being phagocytized
ATP
which of the following is not part of the passive transport process
lophotrichous flagella
which of the following terms best describes the cell in figure 4.2
chromatophores
in bacteria, photosynthetic pigments are found in
requires transporter proteins
the difference between simple diffusion and facilitated diffusion is that facilitated diffusion
taxic movements of the cell in response to attractants or repellents
the term "run" and "tumble" are generally associated with
has a cell wall
You have isolated a motile, gram-positive cell with no visible nucleus. you can safely assume that the cell
pili are used to transfer DNA and motility
Fimbriae and pili differ in that
B
Which diagram of a cell wall is gram-negative cell wall
B
which diagram of a cell wall possesses lipid A/endotoxin responsible for symptoms associated with infection
both A and B
which diagram of a cell wall has a structure that protects against osmotic lysis
B
which diagram of a cell wall is decolorized by alcohol
B
which diagram of a cell wall is resistant to many antibiotics
A
which diagram of a cell wall contains teichoic acids
A
which diagram of a cell wall contains porins
the plasma membrane
where are phospholipids most likely found in a prokaryotic cell
the plasma membrane, around organelles, and surrounding flagella
where are phospholipids most likely found in a eukaryotic cell
cell wall
which of the following is not found or observed to occur in both mitochondria and prokaryotes
it involves movement of molecules down a concentration gradient and may require a transport protein
which of the following statements is correct about passive diffusion
thorugh simple diffusion
oxygen crosses a plasma membrane
plasmolyze
in a hypertonic solution, a bacterial cell will typically
the cell will undergo osmotic lysis
what will happen if a bacteriacel is pretreated with a lysozyme solution, then placed in distilled water
spirilla have an external flagella but spirochetes have axial filaments
how do spirochetes and spirilla differ
ribosomes - carbon storage
which one of the following pairs is mismatched
carboxysomes
which of the following are not energy reseves
cilia - pilia
Which of the following is not a functionally analogous pair
is circular in structure
the DNA found in most bacterial cells
true
the cell walls of bacteria are responsible for the shape of the bacteria and the difference in the gram stain reaction
true
antibiotics that target the cell wall are an effective treatment against many pathogenic bacteria
false
cells placed in a hypotonic solution tend to lose water due to osmotic pressure
true
small, hydrophobic molecules pass through a cell membrane most easily by diffusion
true
spheroplasts, protoplasts, and mycoplasms are bacterial cells without cell walls
false
endospores are a reproductive structure
true
the internal structure of eukaryotic cilia and flagella are the same
false
many enzymes in both prokaryotic and eukaryotic cells are compartmentalized within organelles
true
the number of organelles such as chloroplasts, mitochondria, and rough endoplasmic reticulum is the same in all eukaryotic cells
true
if you observe rod-shaped red cells after the gram stain, you can assume their cell walls contain endotoxin
prokaryotic cells' DNA is not enclosed within a membrane and is usually single.they usually lack organelles and their cell walls contain peptidoglycan. Eukaryotic cells' DNA is found in the cell's nucleus, they have a number os membrane-enclosed organelles and their cell walls are chemically simple
characteristics of a typical prokaryotic and eukaryotic cell
the application of alcohol dehydrates the peptidoglycan of gram positve to make it more impermeable to crystals violet iodine. in gram negative, alcohol dissolves the outer membrane and leaves small holes in the thin peptidoglycan layer through which crystal violet-iodine diffuse. Gram-positive cell walls consist of peptidoglycan while gram-negative contains a few layers of peptidoglycan and anouter membrane
compare and contrast gram-positive and gram-negative cell walls
a complex aggregation of microbes. microorganisms exists as single cells that float or swim independently on a liquid or attach to each other and some solid surface. it can cause infections and its bacteria are often resistant to antibiot
What is a biofilm and what role does biofilm play in disease
remains in pairs after dividing
dipploccoci
divides and remain attached in chain-like patterns
streptococci
vibrios - curved rods
spirillum - helical shape
spirochete - helical and flexible
spiral bacteria
the structure of a prokaryotic cell
capsule
fimbriae
chromosome
pilus
flagella
arrangements of bacterial flagella
2 basal body
gram positive bacterium
4 basal bodies
gram negative bacterium
gram positive cell wall
gram negative cell wall
DNA is not enclosed withing a membrane, it is inside the cell
DNA is not associated with histones
Lack organelles
cell walls contain peptidoglycan
divide by binary fission
prokaryotes
DNA found in cell's nucleus
DNA consistently associated with histones and non histones
Have membrane -enclosed organelles
cell walls are chemically simple
cell division involves mitosis
eukaryotes
cocci - round shape
bacilli -rod-like
spiral
arrangement of cells
maintains a single shape
monomorphic
can have many shapes
pleomorphic
viscous, gelatinous sugar
glycocalyx
a glycocalyx that helps cells in a biofilm attach to their target environment and to each other
extracellular polymeric substance
capsule is neatly organized and firmly attached while slime is unorganized and loose
what is the difference between capsule and slime
bundles of fibrils that arise at the ends of the cell.
axial filament/endoflagella
axial filaments
spirochete move by the means of
helps bacterias attach to the wall
can occur at the poles of the bacterial cell or can be evenly distributed over the entire surface of the cell
fimbriae
longer than fimbriae and only one or two per cell. use to transfer plasmid
pili
primary stain. Stains both gram positive/negative purple
crystal violet
forms large crystals with the dye that are too large to escape through the cell wall
Iodine
it makes gram positive more impermeable to crystal violet-iodine
what happens to gram positive when alcohol is applied
alcohol dissolves the outermembrane and leaves holes for crystal violet to diffuse
what happens to gram negative when alcohol is applied
purple
what color does gram positive turn when safranin is applied
red
what color does gram negative turn when safranin is applied
plasma membrane have sterols (lipids). protects them from lysis (rupture)
atypical cell walls
used to identify mycobacterium and norcardia
bacteria contain mycolic acid in their cell wall
can be stained with carbolfuchsin
acid- fast cell walls
wall-less cell
protoplast
remaining outer wall layer
spheroplast
contains bacterial chromosome
the cell's genetic information which carries all the information required for the cell's structures and functions.
Nucleoid
double stranded DNA molecules. replicate independently of chromosomal DNA
plasmid
protein synthesis take place
prokaryotic ribosome - 70s ribosomes
eukaryotic ribosome - 80s
50s, 30s
ribosomes